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叶绿素缺乏的大麦突变体PSⅡ光化学效率较高的原因

谭新星, 许大全, 汤泽生

中科院上海植物生理研究所.上海 200032；四川师范学院生物系.四川,南充 637002

摘要

在相同的叶绿素浓度,叶绿素缺乏的大麦突变体的叶绿体在450-480nm和600-640nm波长范围的光吸收值比野生型略高,而在400-440nm和700-740nm波长范围的光吸收值明显高于野生型;突变体叶片和叶绿体的低温(77K)荧光发射强度较低,而两个光系低温荧光产量的比值(F685/F735)较高;失去Mg~(2+)后,突变体和野生型的F685/F735和Fv/Fm均降低,但突变体的降低幅度较小;突变体的叶绿体中基粒片层数目较少、长度较短,而间质片层较长。

关键词： PSⅡ光化学效率; 激发能转移; 叶绿素荧光; 叶绿素缺乏的突变体; 大麦

A cause of higher photochemical efficiency of photosystem II in a chlorophyll-deficient barley mutant

Tan Xinxing, Xu Daquan, Tang Zesheng

Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032；China

Abstract

As compared with the wild-type, the light absorption of chloroplasts in mutant barley was slightly higher within 450-480 nm and 600-640 nm but much higher within 400-440 nm and 700-740 nm in the case of equal amount of chlorophyll. The mutant had a lower chlorophyll fluorescence yield at low temperature (77K), but a higher ratio of fluorescence yield of PS II to that of PS I, F685/F735. The effects of Mg~(2+) depletion, which could lower the F685/F735 ratio and the photochemical efficiency of PS II, Fv/Fm, were weaker in the mutant than in the wild-type. Marked changes in chloroplast ultrastructure, such as fewer grana stacks and longer stromal lamellae, were observed in the mutant.

Key words: PS Ⅱ photochemical efficiency;Excitation energy transfer;Chlorophyll fluorescence;Chlorophyll-deficient mutant;Barley

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引用本文：

谭新星, 许大全, 汤泽生. 叶绿素缺乏的大麦突变体PSⅡ光化学效率较高的原因[J]. 生理学报 1997; 49 (3): .

Tan Xinxing, Xu Daquan, Tang Zesheng. A cause of higher photochemical efficiency of photosystem II in a chlorophyll-deficient barley mutant. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).