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病原激发子对番茄阴离子过氧化物酶表达与反应性氧迸发的诱导

刘曼西, Kolattukudy P E

华中理工大学生物工程系.湖北,武汉 430074；俄亥俄州立大学生物工程中心.俄亥俄

摘要

采用Northern印迹法和鲁米诺化学发光法分析抗性的、近等位基因的敏感番茄细胞株阴离子过氧化物酶基因的转录和细胞反应性氧变化,发现病原真菌激发子和外源H_(2)O_(2)均能促进抗性番茄细胞中阴离子过氧化物酶转录。激发子还能刺激抗性细胞中反应性氧水平暂时急剧升高。敏感番茄细胞对激发子或H_(2)O_(2)没有响应。Ca~(2+)和磷脂酶C的抑制剂硫酸新霉素分别促进和抑制激发子诱导下抗性细胞反应性氧增加。

关键词： 反应性氧; 阴离子过氧化物酶; 激发子; 番茄细胞

Expression of an anionic peroxidase and oxidative burst in tomato cells induced by the elicitor from Verticillium alboatrum

Liu Manxi, Kolattukudy P E

Biotechnology Department,Huazhong University of Science and Technology.Wuhan 430074,Hubei

Abstract

The components of the reactive oxygen species of the oxidative burst might depend on the medium. A large amount of ·OH may appear during oxidate burst if mineral ions are absent in the medium. The oxidative burst may be the product of interactions between the plant and an incompatible pathogen and leads to transient oxidative stress inside the cell which can trigger the expression of some genes such as anionic peroxidase genes, in which Ca~(2+) and inositol phosphate may be involved.

Key words: Reactive oxygen;Anionic peroxidase;Elicitor;Tomato cell

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引用本文：

刘曼西, Kolattukudy P E. 病原激发子对番茄阴离子过氧化物酶表达与反应性氧迸发的诱导[J]. 生理学报 1997; 49 (3): .

Liu Manxi, Kolattukudy P E. Expression of an anionic peroxidase and oxidative burst in tomato cells induced by the elicitor from Verticillium alboatrum. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).