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转绿型白化突变系W_(25)转绿过程中幼苗叶片内碳水化合物和氨基酸组分的变化

吴殿星, 舒庆尧, 夏英武, 夏建峰, 郑星勤, 刘贵付

浙江农业大学原子核农业科学研究所.浙江,杭州 310029

摘要

水稻转绿型白化突变系W_(25)与亲本2177s苗期叶片内碳水化合物总量及蛋白质含量差异明显。亲本2177s幼苗叶片内碳水化合物和蛋白质代谢在两种温度下基本稳定。但W_(25)却有明显不同,25℃下W_(25)叶片内蔗糖和淀粉的含量比20℃下的低,但还原糖含量明显地高,蛋白质含量较高,丙酮酸激酶和谷氨酰胺合成酶活性强,游离氨基酸中Ser、Glu、Val的含量明显较低,Arg、Pro、Thr含量明显较高,尤其是Thr含量接近了亲本水平。

关键词： 转绿型白化突变系; 水稻; 温度; 碳水化合物; 蛋白质

Carbohydrate and protein metabolism in seedling leaves of a greenable albino mutant line cv. W_(25) of rice (Oryza sativa)

Wu Dianxing, Shu Qingyao, Xia Yingwu, Xia Jianfeng, Zhen Xingqin, Liu Guifu

Institute of Nuclear Agricultural Sciences,Zhejiang Agricultural University.Hangzhou 310029,Zhejiang

Abstract

Significant differences in carbohydrate content and protein content were found between seedling leaves of W_(25) and its parent line 2177s (Figs. 1, 2). The contents of carbohydrates and proteins in leaves of 2177s were the same under 20 degree C and under 25 degree C, but were obviously different in leaves of W_(25), where the contents of starch, sucrose and free amino acids grown under 25 degree C were significantly lower than those of 20 degree C, while the contents of reducing sugar and protein were the other way round. The contents of free amino acids were about the same in leaves of W_(25) grown under 25 degree C and under 20 degree C, except that Ser, Glu and Val were lower under 25 degree C than under 20 degree C, and those of Arg, Pro and Thr were higher under 25 degree C than under 20 degree C(Fig. 3). The activities of pyruvate kinase and glutamine synthetase in leaves of W_(25) were also higher under 25 degree C than under 20 degree C (Figs. 4,5).

Key words: Greenable albino mutant line;Rice;Temperature;Carbohydrate;Protein

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引用本文：

吴殿星, 舒庆尧, 夏英武, 夏建峰, 郑星勤, 刘贵付. 转绿型白化突变系W_(25)转绿过程中幼苗叶片内碳水化合物和氨基酸组分的变化[J]. 生理学报 1997; 49 (3): .

Wu Dianxing, Shu Qingyao, Xia Yingwu, Xia Jianfeng, Zhen Xingqin, Liu Guifu. Carbohydrate and protein metabolism in seedling leaves of a greenable albino mutant line cv. W_(25) of rice (Oryza sativa). Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).