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水稻蜡质基因5^非翻译区一个与调控有关的内含子

蔡秀玲, 王宗阳, 郑霏琴, 洪孟民

中科院上海植物生理研究所.上海 200032

摘要

从发育的水稻种子中分离出RNA,经RT-PCR反应并结合顺序测定,在籼稻232蜡质基因编码区5^上游非翻译区中证明确实存在一个长度为1126bp的内含子,其A+T碱基的含量高达67.4%,它的边界符合真核基因内含子的GT-AG规则。表明该内含子与蜡质基因的表达调控有一定的关系。

关键词： 第1内含子; 蜡质基因; 水稻

A regulation-related intron in 5^ untranslated region of rice waxy gene

Cai Xiuling, Wang Zongyang, Zheng Feiqin, Hong Mengmin

Shanghai Institute of Plant Physiology,The Chinese Academy of Science.Shanghai 200032

Abstract

Using the methods of polymerase chain reaction-amplified reverse-transcribed RNA isolated from the developing seeds and sequence analysis of amplified product, an intron of 1126 bp in size was identified in the 5^-untranslated region of rice Oryza sativa subsp. indica 232 cultivar Wx gene (Figs 2, 3). Its A+T content was found to be 67.4% (Table 1). The border sequences of this intron conform to the GT-AG rule. Considering similar results of previous experiments, it is suggested that this intron may play an important role in the regulation of Wx gene expression.

Key words: Nitron 1;Wx gene;Rice

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引用本文：

蔡秀玲, 王宗阳, 郑霏琴, 洪孟民. 水稻蜡质基因5^非翻译区一个与调控有关的内含子[J]. 生理学报 1997; 49 (3): .

Cai Xiuling, Wang Zongyang, Zheng Feiqin, Hong Mengmin. A regulation-related intron in 5^ untranslated region of rice waxy gene. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).