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红光和 Ca~(2+)对与绿豆下胚轴伸长有关的细胞壁酶的影响

龙程, 张志良, 沈曾佑, 颜季琼

华东师范大学生物系. 上海　200062

摘要

关键词： β-1; Ca~(2+); 绿豆下胚轴; 伸长; 过氧化物酶; 红光; 3-葡聚精酶; 4-葡聚糖酶

Effect of red light and Ca~(2+) on cell wall enzyme activities associated with elongation of hypocotyls of Phaseolus radiatus L.

Long Cheng, Zhang Zhiliang, Seng Zengyou, Yan Jiqiong

Department of Biology, East China Normal University. Shanghai 200062

Abstract

In darkness, segments of hypocotyls from etiolated Phaseolus radiatus L. seedlings showed high growth rate in Ca~(2+)-free medium, but their elongation was inhibited when Ca~(2+) (1 mmol/L) was applied. Red light inhibited the elongation of segments with or without Ca~(2+) in the medium, and the inhibition was more significant when Ca~(2+) was present. We found that the activity of covalently bound peroxidase strongly increased, the activities of covalently bound #beta#-1,4-glcanase and #beta#-1,3-glcanase markedly decreased during inhibition of elongation. These findings indicated that these enzymes were responsible for hypocotyl elongation. Profound changes occurred during the time 2-4 h after darkness or red light treatment. Otherwise, the changes in enzyme activity of cell wall would be more noticeable when hypocotyls were treated by a combination of red light and Ca~(2+) than by either red light or Ca~(2+) alone.

Key words: #beta#-1;Ca~(2+);Phaseolus radiatus;Hypocotyl;Elongation Peroxidase;Red light;3-glucanase;4-glucanase

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引用本文：

龙程, 张志良, 沈曾佑, 颜季琼. 红光和 Ca~(2+)对与绿豆下胚轴伸长有关的细胞壁酶的影响[J]. 生理学报 1997; 49 (2): .

Long Cheng, Zhang Zhiliang, Seng Zengyou, Yan Jiqiong. Effect of red light and Ca~(2+) on cell wall enzyme activities associated with elongation of hypocotyls of Phaseolus radiatus L.. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).