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4a4PU-30对水稻叶片衰老与内源激素的调控

汤日圣, 梅传生, 陈以峰, 吴光南

江苏省农业科学院农业生物遗传生理研究所. 江苏, 南京　210014

摘要

4PU-30能显著地延缓水稻叶片衰老。根据叶片衰老过程中内源激素含量的变化, 可明确减缓水稻叶片衰老期间内源ABA含量的增加和内源ZRs、GAs和IAA含量的减少,使叶片中保持有较低水平的ABA与较高水平的ZRs、GAs和IAA,是4PU-30延缓水稻叶片衰老的主要调控机理。

关键词： 4PU-30; 内源激素; 叶片衰老; 水稻

The regulation of 4PU-30 on senescence of riceleaves and endogenous hormones

Tang Risheng, Mei Chuansheng, Chen Yifeng, Wu Guangnan

Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences. Nanjing 210014, Jiangsu

Abstract

Effects of 4PU-30〔N-phenyl-N^-(2-chloro-4-pyridyl) urea〕on senescence of rice leaves and contents of endogenous hormones were investigated. ELISA (enzyme-linked immune sorbent assay) was used to determine the contents of endogenous hormones. In the experiments with detached leaves: The top two fully developed leaves were collected during heading and cut into 5 cm segments, then floated on the surfaces of 4PU-30 at concentrations 0.004,0.04,0.4 and 4.0 mu mol/L respectively and incubated in darkness at 30℃. The results showed that the progress of leaf senescence was retarded significantly at all concentrations, meanwhile, the increase of content of endogenous ABA and the decrease of contents of endogenous ZRs, GAs and IAA were all retarded. In the experiment with attached leaves, rice plant were sprayed with 115 ml/m2 4PU-30 (4 mu mol/L) solution 10　d after heading, and then sprayed at one week intervals twice. The results showed that senescence of attached rice leaves was delayed significantly by 4PU-30, and the increase of the content of endogenous ABA and the decreases of the contents of endogenous ZRs,GAs and IAA were all slowed down as compare with the control. Therefore, it was proposed that the mechanism of action of 4PU-30 in delaying the senescence of rice leaves is through the regulation of the change in concentration of endogenous plant hormones in leaves, i.e, by delaying the increase of ABA content and the decreases of ZRs, GAs and IAA contents in rice leaves.

Key words: 4PU-30;Endogenous hormones;Leaf senescence;Rice

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引用本文：

汤日圣, 梅传生, 陈以峰, 吴光南. 4a4PU-30对水稻叶片衰老与内源激素的调控[J]. 生理学报 1997; 49 (2): .

Tang Risheng, Mei Chuansheng, Chen Yifeng, Wu Guangnan. The regulation of 4PU-30 on senescence of riceleaves and endogenous hormones. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).