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黄皮种子脱水敏感性与脂质过氧化作用

宋松泉, 傅家瑞

中山大学生命科学学院. 广东， 广州 510275

摘要

黄皮种子自然脱水时，种子的发芽率和发芽指数迅速下降；种子浸泡液的电导率和可溶性物质的量大大增加；线粒体膜和质膜ATPase的活性下降；种子中SOD活性先上升，然后下降；脂质过氧化产物MDA和脂质氢过氧化物的量大大增加。DDC、MDA和Fe~(2+)促进脂质过氧化作用，降低种子生活力；AsA和甘露醇对脂质过氧化有抑制作用，提高种子生活力。

关键词： 顽拗性种子; 脱水敏感性; 脂质过氧化作用; 黄皮种子

Desiccation-sensitivity and lipid peroxidation in Chinese wampee[Clausena lansium（Lour.）Skeels] seeds

Song Songquan, Fu Jiarui

School of Life Sciences,Zhongshan University. Guangzhou 510275, Guangdong

Abstract

Chinese wampee seeds which are very sensitive to desiccation are typical recalcitrant seeds.The germination percentage and germination index of seeds fell to zero when the moisture content decreased to 22.4% from the original average of 51% .When the seeds were first allowed to undergo natural desiccation，the amount of soluble substance leaking out of the seeds into the water in which the Chinese wampee seeds were soaked was higher.In the meantime,ATPase activities in mitochondrial membrane and plasma membrane decreased, and SOD activity in seeds rose at first, andthen declined, and the products of lipid peroxidation, malondialdehyde(MDA)and lipid hydroperoxides increased markedly.Diethyldithiocarbamic acid, MDA and Fe~(2+) promoted lipid peroxidation, and lowered seed viability.Ascorbic acid and mannitol retarded lipid peroxidation,and improved seed viability.

Key words: Recalcitrant seed;Desiccation-sensitivity;Lipid Peroxidation;Clausean lansium (Lour.)

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引用本文：

宋松泉, 傅家瑞. 黄皮种子脱水敏感性与脂质过氧化作用[J]. 生理学报 1997; 49 (2): .

Song Songquan, Fu Jiarui. Desiccation-sensitivity and lipid peroxidation in Chinese wampee[Clausena lansium（Lour.）Skeels] seeds. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).