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红豆草抗羟脯氨酸变异系原生质体培养后愈伤组织的抗性特征

徐子勤, 贾敬芬

兰州大学细胞生物学研究室.甘肃, 兰州 730000

摘要

通过NaN_(3)诱变得到的红豆草抗羟脯氨酸(Hyp)变异系,在酶液中游离原生质体进行培养,获得再生植株。 在含不同浓度NaCl、羟脯氨酸或PEG的MS培养基上,原生质体来源抗性愈伤组织中的游离脯氨酸含量在1wk之内均急剧增加,随后开始下降, 3wk后接近正常水平。随着胁迫程度的提高,抗性愈伤组织中游离脯氨酸含量呈递增趋势,生长速度呈递减趋势。

关键词： 红豆草; 羟脯氨酸(Hyp); 游离脯氨酸; 原生质体; 抗性; 稳定遗传; 渗透调节

Resistant characteristics of the hydroxyproline resistant cell line in Onobrychis viciaefolia after protoplast culture

Xu Ziqin, Jia Jingfen

Cell Biology Laboratory of Lanzhou University. Lanzhou 730000, Gansu

Abstract

The hydroxyproline resistant cell line of Onobrychis viciaefolia had been　selected through NaN_(3) mutagenesis. Protoplast-derived plantlets of the　resistant cell line were regenerated in four months and were found to retain　strong hydroxyproline resistance. After the calli of the resistant cell line　derived from protoplasts were treated with different concentrations of NaCl,Hyp or PEG, the free proline content increased steeply in the first week and　descended to normal level in three weeks. In contrast to this, proline　accumulation in the wild tissue was not found at low selection level it could　stand. Along with the enhancement of stress, the free proline concentration in　calli of the resistant cell line increased, while the relative growth rate　descended. When the resistant cell line was transferred from selection medium onto normal MS medium, its free proline content declined　to the normal level in six days. It suggested that free proline　accumulation was related to osmotic regulation and was the major way to　prevent water loss of the resistant cell line under water stress. Total amino　acid analysis revealed that methionine content of the regenerated plantlet　leaves derived from hydroxyproline-resistant protoplasts was twice as high as the original type.

Key words: Onobrychis viciaefolia;Hydroxyproline;Free proline;Protoplast;Resistance;Stable inherity;Osmotic regulation

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引用本文：

徐子勤, 贾敬芬. 红豆草抗羟脯氨酸变异系原生质体培养后愈伤组织的抗性特征[J]. 生理学报 1997; 49 (2): .

Xu Ziqin, Jia Jingfen. Resistant characteristics of the hydroxyproline resistant cell line in Onobrychis viciaefolia after protoplast culture. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).