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黄鼠与大鼠离体心脏预缺血处理对缺血再灌损伤的保护作用

高天礼, 黄玉芝

北京大学生命科学学院生理生物物理系.北京 100871

摘要

对照组达乌尔黄鼠和Wistar大鼠离体心脏在第二次缺灌10min期内肌酸激酶(CK)释放均较预缺灌期明显增加,两种动物的增加幅度基本一致,再灌10min期内CK释放也未见明显减小,说明一次性预缺灌15min对第二次缺灌10min未表现保护作用。实验组两种动物在第二次缺灌和再灌10min期内CK释放均较预缺灌期明显下降,且两种动物的下降幅度也基本一致。此外,实验组两种动物的心律失常发生率也都明显低于对照组。两种动物间比较,实验组的黄鼠较大鼠经预缺灌处理后对随后缺血再灌损伤具有更明显的保护作用。

关键词： 预缺血处理; 肌酸激酶; 缺灌损伤; 再灌损伤

Ischemic preconditional protection of ischemia reperfusion injury on isolated hearts of ground squirrel and rat

Gao Tianli, Huang Yuzhi

College of Life Sciences, Peking University. Beijing 100871

Abstract

The protective effects of ischemic precoditioning on ischemia-reperfusion injury was investigated using isolated Langendorff perfusing hearts from ground squirrel and rat. In Preconditioning I group hearts were first perfused wit Krebs-Henseleit solution for 10 min to establish a steady state, then stopped for 15 min to establish global ischemia, and finally followed by 10 min ischemia and 10 min reperfusion. In Preconditioning Ⅱ group there were three cycles of 5 min ischemia +5 min reperfusion after 10 min equilibration and then the final 10 min ischemia and 10 min reperfusion were followed. It was found that in group Ⅰ durign the final 10 min ischemic period there was remarkable augmentation of CK release from both animal^s hearts. But in group Ⅱ CK release decreased markedly during the same ischemic period. CK release during final 10 min reperfusion period also decreased significantly in group Ⅱ in comparison with group Ⅰ. The incidence of arrhythmias occurred in both animal^s hearts was markedly reduced in group Ⅱ rather than group Ⅰ.

Key words: Ischemic preconditioning;Creatine kinase;Ischemic injury;Reperfusion injury

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引用本文：

高天礼, 黄玉芝. 黄鼠与大鼠离体心脏预缺血处理对缺血再灌损伤的保护作用[J]. 生理学报 1997; 49 (2): .

Gao Tianli, Huang Yuzhi. Ischemic preconditional protection of ischemia reperfusion injury on isolated hearts of ground squirrel and rat. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).