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腹侧被盖区DA神经元调节睡眠-觉醒机制的探讨

王志, 林殷利

上海第二医科大学生理教研室.上海 200025

摘要

大鼠中脑腹侧被盖区(VTA)注射GABA(25#mu#g)和5-HT(2 #mu#g),伏隔核(Acb)内多巴胺(DA)代谢产物--双羟苯乙酸(DOPAC)分别降低到注射前的68.2％(P<0.01)和升高到136.1％(P<0.01),并相应减少和增加觉醒。双侧Acb注射DA(10#mu#g),也可明显增加觉醒,此作用可被氟哌啶醇(16pmol)阻断,SCH23390不起作用。

关键词： γ-氨基丁酸; 5-羟色胺; 腹侧被盖区; 优隔核; 睡眠-觉醒; 3 4-双羟苯乙酸

Regulation of dopamine neurons in ventral tegmental area on sleep-wakefulness

Wang Zhi, Lin Yinli

Department of Physiology, Shanghai Second Medical University. Shanghai 200025

Abstract

The effects of microinjection of #gamma#-aminobutyric acid (GABA) and serotonin (5-HT) into the ventral tegmental area (VTA) on the activity of dopamine neurons in VTA and sleep-wakefulness were studied in rats. It was observed that in aneasthetized rats microinjection of GABA (25 #mu#g) and 5-HT (2 #mu#g) respectively decreased or incrased the level of 3, 4-dihydroxyphenylacetic acid (DOPAC) to 68.2% (P<0.01) and to 136.1% (P<0.01) of control value in the nucleus accumbens (Acb). It was also observed that in free moving rats the time of wakefulness was respectively decreased and increased by the two drugs. Furthermore, the time of wakefulness was enhanced following microinjection of dopamine (10 #mu#g) into Acb, an effect that could be blocked by haloperidal (16 pmol), but not by SCH23390 (2 nmol).

Key words: #gamma#-aminobutyric acid;Serotonin;Ventral tegmental area;Nucleus accumbens;Sleepwakefulness;3, 4 -dihydroxyphenylacetic acid

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引用本文：

王志, 林殷利. 腹侧被盖区DA神经元调节睡眠-觉醒机制的探讨[J]. 生理学报 1997; 49 (2): .

Wang Zhi, Lin Yinli. Regulation of dopamine neurons in ventral tegmental area on sleep-wakefulness. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).