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白细胞介素-2对大鼠离体垂体前叶细胞增殖的影响

王高峰, 朱运龙, 胡玉珍, 张万会

第四军医大学生理教研室.陕西,西安 710032

摘要

IL-2(10-500U/ml)明显促进雌性大鼠包括妊娠大鼠的垂体前叶(AP)细胞的增殖,而抑制雄性大鼠AP细胞的增殖。雌性大鼠行卵巢切除(OVX)2wk后,IL-2对其AP细胞增殖的影响反转为抑制效应;若在卵巢切除2wk内,每日给予OVX大鼠sc5#mu#g苯甲酸雌二醇,IL-2的促增殖效应可基本恢复。雄性大鼠行睾丸切除术2wk后,IL-2对其AP细胞的抑制效应消失,但并不发生反转。

关键词： 白组胞介素-2; 垂体前叶; 细胞增殖; 3H－TdR修入; 雌二醇; 大鼠

Effect of interleukin-2 on the proliferation of cultured rat anterior pituitary cells

Wang Gaofeng, Zhu Yunlong, Hu Yuzhen, Zhang Wanhui

The Fouth Military Medical University. Xi^an 710032, Shaanxi

Abstract

The present study was to investigate whether IL-2 is involved in the proliferation control of anterior pituitary (AP) cells. Primary culture of AP cells was obtained from either male or female Sprague-Dawley rats. The effects of IL-2 were assessed by ~(3)Hthymidine incorporation rate. It was found that: (1) IL-2 (10-500 U/ml) significantly stimulated ~(3)H-TdR incorporation into AP cells of female pregnant as well as nont-pregnant rats, but inhibited ~(3)H-TdR incorporation in the male rats. (2) The stimulatory effect of IL-2 was reversed in overiectomized rats, but could be restored when the overiectomized rats were treated with estradiol benzoat (5 #mu#g/d per rat). (3) When the male rats were orchiectomized for 2 weeks, the inhibitory effects of IL-2 did not occur.

Key words: Interleukin-2;Anterior pituitary;Cell growth;3H-TdR;Incorporation estradiol;Rat

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引用本文：

王高峰, 朱运龙, 胡玉珍, 张万会. 白细胞介素-2对大鼠离体垂体前叶细胞增殖的影响[J]. 生理学报 1997; 49 (2): .

Wang Gaofeng, Zhu Yunlong, Hu Yuzhen, Zhang Wanhui. Effect of interleukin-2 on the proliferation of cultured rat anterior pituitary cells. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).