过刊浏览

基因重组白细胞介素3刺激胎肝巨核细胞祖细胞生长的特性

马东初, 孙英慧, 常奎忠

沈阳军区总医院医学实验科.辽宁,沈阳 110015

摘要

采用血浆凝块、甲基纤维素半固体和液体培养体系结合流式细胞仪巨核细胞DNA双荧光分析,观察并研究了4-5个月胎肝和成人骨髓CFU-MK在体外的增殖和分化特点。

关键词： 巨核细胞; DNA倍性; CFU－MK; 胎肝

In vitro growth characteristics of rIL_(3) stimulated megakaryocytic progenitor cells (CFU-MK) or fetal liver

Ma Dongchu, Sun Yinghui, Chang Kuizhong

Department of Experimental Medicine, General Hospital of Shenyang. Shenyang 110015, Liaoning

Abstract

In this study, plasma clot and methycellulose semi-solid and liquid culture technigues were employed to observe the in vitro growth characteristics of proliferation and differetiation of 4-5 months fetal liver and adult bone marrow CFU-MKs. Fetal liver MK colonies in plasma clot in the presence of rIL_(3) or Meg-CSA were larger (usually with >50 cells/colony) than that of the adult (usually with<50 cells/colony).

Key words: Megakaryocytes;DNA ploidy;CFU-MK;fetal liver

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

马东初, 孙英慧, 常奎忠. 基因重组白细胞介素3刺激胎肝巨核细胞祖细胞生长的特性[J]. 生理学报 1997; 49 (2): .

Ma Dongchu, Sun Yinghui, Chang Kuizhong. In vitro growth characteristics of rIL_(3) stimulated megakaryocytic progenitor cells (CFU-MK) or fetal liver. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).