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异丙酚抑制大鼠心室肌细胞短暂外向钾电流

周军, 田鸣, 周兆年

中科院上海生理研究所.上海 200031；徐州医学院麻醉生理研究所.江苏,徐州 221002

摘要

利用全细胞膜片箝技术研究了异丙酚25,50 #mu#mol/L 对离体大鼠心室肌细胞短暂外向钾通道电流(I_(to))的影响。观察到用药后I_(to)的幅度明显减小,而用不含药物的灌流液冲洗细胞,可使受抑电流部分恢复。当膜电位大于0 mV时,异丙酚对I_(to)抑制达显著水平,但不改变其电压依赖性和I-V关系中的外向整流特性。

关键词： 异丙酚; 全细胞膜片箝技术; 短暂外向钾电流

Inhibition of transient outward potassium current in rat ventricular myocytes by propofol

Zhou Jun, Tian Ming, Zhou Zhaonian

Shanghai Institute of Physiology, Chinese Academy of Sciences.Shanghai 200031；China

Abstract

Whole-cell patch-clamp technique was applied to investigate the effect of propofol (25,50 #mu#mol/L) on transient outward potassium current (I_(to)) in rat ventricular myocytes. It was shown that the amplitude of I_(to) was decreased significantly after exposure to propofol. The effect is partially reversible after washing. At applied potential≥0 mV, statistical significant differences reached between propofol(25 and 50 #mu#mol/L) group and the control group could be found. The voltage-dependent manner and the outward rectifier character were not affected by the drug at the two concentrations.

Key words: Propofol;Whole-cell patch-clamp technique;Transient outward potassium current

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引用本文：

周军, 田鸣, 周兆年. 异丙酚抑制大鼠心室肌细胞短暂外向钾电流[J]. 生理学报 1997; 49 (1): .

Zhou Jun, Tian Ming, Zhou Zhaonian. Inhibition of transient outward potassium current in rat ventricular myocytes by propofol. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).