过刊浏览

氟哌啶醇和R(-)-NPA抑制C_(6)神经胶质瘤细胞的钾电流

谢重, 何淑舫

中科院上海脑研究所.上海 200031

摘要

氟哌啶醇(HALO)和R(-)-Propylnorapomorphine(NPA)能抑制C_(6)细胞的K~(+)电流,其抑制作用是可逆的,而且都与药物浓度有依赖关系。两者所抑制的主要是K~(+)电流中的慢成分,而对快成分的作用有所不同。它们的抑制作用不是由多巴胺D_(2)受体介导的,也不是通过G-蛋白或细胞内钙作用的,很可能是直接作用于钾通道。

Inhibition of voltage-dependent K~(+) currents in rat C_(6) glioma cells by haloperidol and R(-)-NPA

Xie Zhong, He Shufang

Shanghai Brain Research Institute, Chinese Academy of Sciences. Shanghai 200031

Abstract

In this study, the inhibitory effects of R (-) -NPA (R (-) - Propylnorapomorphine) and haloperidol on voltage-dependent K~(+) currents in rat C_(6) glioma cells (C_(6) cells) were observed by means of the whole-cell patch-clamp recording technique. The mechanisms of the effects were also analyzed.The main results were as follows:(1) Both R (-) -NPA and haloperidol could inhibit K~(+) currents dose-dependently and reversibly in C_(6) cells. (2) R (-) -NPA and haloperidol inhibited mainly the slow component of the K~(+) currents. (3)These inhibitory responses were not mediated by dopamine receptors or dependent on G-proteins and intracellular calcium. It was suggested that they refer to some direct action on K~(+) channels.

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

谢重, 何淑舫. 氟哌啶醇和R(-)-NPA抑制C_(6)神经胶质瘤细胞的钾电流[J]. 生理学报 1997; 49 (1): .

Xie Zhong, He Shufang. Inhibition of voltage-dependent K~(+) currents in rat C_(6) glioma cells by haloperidol and R(-)-NPA. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).