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自发性高血压大鼠动脉平滑肌细胞内肌醇磷脂代谢的改变

胡申江, 黄元伟

浙江医科大学附属第一医院心内科.浙江,杭州 310003

摘要

在基础状态下自发性高血压大鼠动脉平滑肌细胞内三磷酸肌醇的含量基本正常;GTR#gamma#S激活G蛋白后,动脉平滑肌细胞三磷酸肌醇的增加呈剂量和时间依赖性;G蛋白的持续激活使自发性高血压大鼠的三磷酸肌醇的增量显著大于Wistar-Kyoto对照组大鼠。

关键词： 自发性高血压大鼠; 动脉平滑肌细胞; G蛋白; 肌醇确脂

Alteration of inositol phosphate metabolism in aortic smooth muscle cells from spontaneously hypertensive rat

Hu Shenjiang, Huang Yuanwei

Cardiovascular Department, the First Affiliated Hospital, Zhejiang Medical University. Hangzhou 310003；China

Abstract

The changes of inositol phosphates in quiescent and GTR_(#gamma#)S activated vascular smooth muscle cells (VSMC) derived from aorta of spontaneously hypertensive rat (SHR) were examined. The inositol phosphate assay revealed that there was mo difference in the inositol-1,4,5-triphosphate (InsP_(3)) level between the quiescent VSMC_(s) of SHR and that of normotensive Wistar-Kyoto rats (WKY). G-protein activator GTR_(#gamma#)S caused a dose-and time-dependent increase in the InsP_(3) level in VSMC_(s). Activated G proteins increased significantly the InsP_(3) level in VSMC_(s) of SHR. In conclusion, the highly sensitive reactivity to GTR_(#gamma#)S in the generationof InsP_(3) in VSMC_(s) from SHR may be related to the possible abnomality of the number, substructure or function of some components in G proteins, phosphoinositide turnover.

Key words: Spontaneously hypertensive rat;Vascular smooth muscle cells;G protein;Inositol phosphates

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引用本文：

胡申江, 黄元伟. 自发性高血压大鼠动脉平滑肌细胞内肌醇磷脂代谢的改变[J]. 生理学报 1997; 49 (1): .

Hu Shenjiang, Huang Yuanwei. Alteration of inositol phosphate metabolism in aortic smooth muscle cells from spontaneously hypertensive rat. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).