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GRP和VIP对E_(2)诱致的垂体催乳素(PRL)瘤细胞PRL基因转录的影响

狄安稞, 单惠敏, 黄曼影, 许荣kun

大连医科大学.辽宁,大连 116023；医科院基础医学研究所、中国协和医科大学基础医学院生理研究室。北京 100005

摘要

不同剂量的胃泌素释放肽(GRP)和血管活性肠肽(VIP)分别与PRL瘤细胞孵育24h后,10~(-8)mol/L、10~(-7)mol/L的GRP均不影响PRL瘤细胞内PRL mRNA 水平,但10~(-6) mol/L的GRP可使胞内PRL mRNA 水平下降20%(P＜0.05)。在实验所采用的浓度范围内,VIP均可升高PRL瘤细胞内PRL mRNA水平,10~(-8)mol/L、10~(-7)mol,10~(-6)mol/L的VIP分别使胞内PRL mRNA 升高为对照组的1.60、2.10、2.21倍(P＜0.05)。三种浓度的E_(2)分别与PRL瘤细胞孵育48h后,10~(-8)mol/L E_(2)不影响细胞内PRL mRNA水平, 但10~(-7)mol/L、10~(-6)mol/L的E_(2)则分别可使胞内PRL mRNA升高为对照组的2.80、2.92倍(P＜0.05)。

关键词： GRP VIP; E_(2); PRL瘤; 细胞培养; 原位杂交; 基因转录

The effects of gastrin-releasing peptide (GRP) and vasoactive intestinal polypeptide (VIP) on the prolactin (PRL) gene transcription of pitutitary PRL releasing tumor of rat

Di Anke, Shan Huimin, Huang Manying, Xu Rongkun

CMB Visiting Scholar from Department of Physiology, Dalian Medical University.Dalian 116023,Liaoning；China

Abstract

The effects of gastrin-releasing peptide (GRP) and vasoactive intestinal polypeptide (VIP) on the prolacitn gene transcription of cultured pituitary of male Sprague-Dawley (SD) rats PRL releasing tumor (PPRT) (induced by estradiol) cells were studied. The PRL mRNA levels were determined by in situ hybridization of cytoplasmic RNA with a DIG-labeled PRL cDNA probe. PRl mRNA levels didn^t change when the PPRT cells were incubated with 10~(-8) mol/L or 10~(-7) mol/L GRP for 24 h, but decreased by 20% when GRP was increased to 10~(-6) mol/L (P＜0.05).The PRL mRNA level increased to 1.60,2.10,2.21 times of the control group when the PPRT cells were respectively incubated with 10~(-8),10~(-7),10~(-6) mol/L VIP for 24 h (P＜0.05). The PRL mRNA level didn^t change when the PPRT tumor cells were incubatcd with 10~(-8) mol/L E~(2) for 48 h, but did increase to 2.80 and 2.92 times of the control group respectively when 10~(-7) mol/L 10~(-6) mol/L E~(2) were used. The results above indicated that GRP and VIP exert an inhibitory and a stimulatory effect on RPL gene transcription respectively, while the stimulatory action of E~(2) on PRL secretion is a direct one.

Key words: Gastrin-releasing peptide;Vasoactive intestinal polypeptide;Estradiol;Pituitary PRL releasing tumor;Cell culture;In situ hybridhatlon

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引用本文：

狄安稞, 单惠敏, 黄曼影, 许荣kun. GRP和VIP对E_(2)诱致的垂体催乳素(PRL)瘤细胞PRL基因转录的影响[J]. 生理学报 1997; 49 (1): .

Di Anke, Shan Huimin, Huang Manying, Xu Rongkun. The effects of gastrin-releasing peptide (GRP) and vasoactive intestinal polypeptide (VIP) on the prolactin (PRL) gene transcription of pitutitary PRL releasing tumor of rat. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).