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大鼠海马脑片缺氧诱导Ca~(2+)/CaM PKII活性抑制机理的研究

裴林, 纵艳艳, 张磊, 孙亚锋, 张光毅

徐州医学院生物化学与分子生物学研究中心.江苏,徐州 221002

摘要

有钙或无钙培养时Ca~(2+)/CaM PKII活性随缺氧时间的延长均下降,但前者比后者酶活性下降更显著。海马脑片在体外缺氧30 min,谷氨酸在胞外的堆积增加2倍多。单纯过量外源性谷氨酸能引起酶活性显著下降。氯胺酮对缺氧和单纯外源性谷氨酸所诱导的酶活性抑制均有明显的拮抗作用。

关键词： Ca~(2+); 海马脑汁; 缺氧; 氯胺酮; Ca~(2+)／CaM依赖性蛋白激酶II

Studies on mechanism of the inhibition of Ca~(2+)/CaM PKII activity induced by hypoxia in rat hippocampal slices

Pei Lin, Zong Yanyan, Zhang Lei, Sun Yafeng, Zhang Guangyi

The Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College. Xuzhou 221002,Jiangsu

Abstract

The effects of Ca~(2+) and ketamine on Ca~(2+)/CaM PKII activity in rat hippocampal slices under in vitro hypoxic states have been studied and the effect of hypoxia on glutamate accumulation was also investigated. The results were as follows: (1) Ca~(2+)/CaM PKII activity decreased gradually with increasing hypoxic time in culture medium with or without 1.3 Ca~(2+) mmol/L, with the former case being much more pronounced. (2) Extracelluar accumulation of GLU increased about two-fold after hypoxia for 30 min in vitro. (3) When slices were incubated for 30 min under conditions in excess ofexogenous GLU the enzyme activity markedly decreased, a finding suggesting that the inhibition of enzymeactivity induced by hypoxia may result from excitotoxicity. (4) The inhibition of the enzyme activity induced by either hypoxia orexcess exogenous glutamate alone could be antagonized markedly by pretreatment with ketamine, suggesting that the hypoxia induced inhibition of the enzyme activity is mediated by NMDA receptor.

Key words: Ca~(2+);Hippocampal slice;Hypoxia;Ketamine;Ca~（2+）/CaM PK Ⅱ

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引用本文：

裴林, 纵艳艳, 张磊, 孙亚锋, 张光毅. 大鼠海马脑片缺氧诱导Ca~(2+)/CaM PKII活性抑制机理的研究[J]. 生理学报 1997; 49 (1): .

Pei Lin, Zong Yanyan, Zhang Lei, Sun Yafeng, Zhang Guangyi. Studies on mechanism of the inhibition of Ca~(2+)/CaM PKII activity induced by hypoxia in rat hippocampal slices. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).