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自发性高血压大鼠主动脉#alpha#_(1B)及#alpha#_(1D)肾上腺素受体第三细胞内环不存在点突变

许开明, 韩启德

北京医科大学第三医院血管医学研究所.北京 100083

摘要

根据G蛋白偶联受体固有活性假说,推测SHR 主动脉中介导肌醇磷脂水解的主要受体-#alpha#_(1D)和#alpha#_(1B)-肾上腺素受体的第三细胞内环可能存在点突变。该实验通过PCR-SSCP鉴定,表明上述突变并不存在。从而提示SHR主动脉磷酸肌醇蓄积基础水平增加并非因#alpha#_(1D)和#alpha#_(1B)-肾上腺素受体第三细胞内环区域突变所引起的受体固有活性所致。

关键词： 高血压; L-精氨酸; 平滑肌细胞; 脂质体

Absence of point mutation in the third intracellular loop of #alpha#_(1B)-and #alpha#_(1D) adrenergic receptor in the aortae of spontaneously hypertensive rats

Xu Kaming, Han Qide

Institute of Vascular Medicine, Third Hospital, Beijing Medical University.Beijing 100083

Abstract

According to the constitutive activity hypothesis for G-protein coupling receptors, we inferred that point mutations might possibly exsit in the third intracellular loop of #alpha#_(1B)-and/or #alpha#_(1D)-adrenergic receptor (AR), the main subtypes of #alpha#_(1)-AR mediating phosphatidylinositol hydrolysis in SHR aortae. This inference, however, was not demonstrated by the results of our PCR-SSCT analyses, which indicated that the increased basallevel of IPs accumulation in the isolated aortae from SHR does not result from constitutive activity of #alpha#_(1B)-and #alpha#_(1D)-AR induced by ponit mutations of the third intracellular loop of the genes

Key words: Hypertension;L-Arginine;Smooth muscle cells;Liposome

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引用本文：

许开明, 韩启德. 自发性高血压大鼠主动脉#alpha#_(1B)及#alpha#_(1D)肾上腺素受体第三细胞内环不存在点突变[J]. 生理学报 1997; 49 (1): .

Xu Kaming, Han Qide. Absence of point mutation in the third intracellular loop of #alpha#_(1B)-and #alpha#_(1D) adrenergic receptor in the aortae of spontaneously hypertensive rats. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).