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西洋参果实中抑制白菜种子发芽物质的分离与鉴定

黄耀阁, 李向高, 崔树玉, 郑毅男, 韩宝瑞

吉林农业大学中药材学院. 吉林, 长春 130118

摘要

改进了传统的发芽抑制物质的提取、分离与鉴定方法，以系统溶剂法初步提取各组分，通过TLC分离与纯化，用GC-MS联用仪进一步分离鉴定，最后以标准品核对并测定抑制物质的活性及半抑制浓度(IC_(50))。首次从西洋参果实中分离鉴定出水合三氯乙醛、己酸和辛酸3种发芽抑制物质，其半抑制浓度分别为577.6、128.4和87.7#mu#l/L。

关键词： 西洋参; 果实; 抑制物质; 水合三氯乙醛; 己酸; 辛酸

Isolation and identification of germination inhibitors in American ginseng fruit

Huang Yaoge, Li Xianggao, Cui Shuyu, Zheng Yinan, Han Baorui

College of Chinese Medicinal Materials, Jilin Agricultural University. Changchun 130118, Jilin

Abstract

The traditional method of germination inhibitor extraction, isolation and identification was improved in the present study. The inhibitors were preliminarily isolated by using a series of solvents,then separated and purified by TLC, and then further isolated and identified with GC-MS. Finally, the IC_(50)(concentration of 50% inhibition) of the inhibitors were determined by calibration with the standards. Three kinds of inhibitor were extractred, isolated and identified for the first time, from the fruit of American ginseng ( Panax quinquefolium L.). They are chloral hydrate, hexanoic acid and octanoic acid and the IC_(50) are 577.6 #mu#l/L, 128.4 #mu#l/L and 87.7 #mu#l/L, respectively.

Key words: American ginseng (Panax quinquefolium L);Fruit;Inhibitors;Chloral hydrate;Hexanoic acid;Octanoic acid

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引用本文：

黄耀阁, 李向高, 崔树玉, 郑毅男, 韩宝瑞. 西洋参果实中抑制白菜种子发芽物质的分离与鉴定[J]. 生理学报 1997; 49 (1): .

Huang Yaoge, Li Xianggao, Cui Shuyu, Zheng Yinan, Han Baorui. Isolation and identification of germination inhibitors in American ginseng fruit. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).