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Cu~(2+)对蓝藻 Spirulina maxima光合作用的抑制机理

李建宏, 邰子厚, 曾昭琪

南京大学生物科学与技术系. 江苏, 南京 210093；南京大学配位化学国家重点实验室. 江苏, 南京 210093

摘要

Cu~(2+)对 S.maxima的生长和光合放氧均有抑制作用；高浓度Cu~(2+)藻细胞中藻胆体产生的特征光吸收和荧光显著下降，表明藻胆体是Cu~(2+)作用位点之一。Cu~(2+)可促使离体的藻蓝蛋白变性，使其光吸收和荧光减弱、荧光偏振度减小，而别藻蓝蛋白对Cu~(2+)的作用不敏感。根据这些结果推测，Cu~(2+)可能通过对藻胆体中的藻蓝蛋白的作用，抑制藻胆体的光能吸收和传递。

关键词： 极大螺旋藻; 铜离子; 藻胆蛋白; 光合作用

Mechanism of Cu~(2+) inhibiting photosynthesis of Spirulina maxima

Li Jianhong, Tai Zihou, Zeng Zhaoqi

Department of Biology Science and Biotechnology, Nanjing University. Nanjing 210093, Jiangsu； China

Abstract

In the paper, the authors studied the effects of Cu~(2+) on the growth and photosynthesis of blue green algae Spirulina maxima, and on the phycobilisomes(PBS) by absorption spectrum and fluorescence spectrum. These results showed that the concentrations of Cu~(2+) inhibiting algal growth and algal photosynthetic oxygen evolution were 10 #mu#mol L~(-1) and 150 #mu#mol　L~(-1) ， respectivly. Cu~(2+) also decreased the characteristic light absorption density of PBS (Fig.3). The changes of fluorescence intensities of PBS caused by different Cu~(2+) concentrations were observed (Fig.4). These results indicated that the PBS was a site of Cu~(2+) acting on. In vitro, Cu~(2+) could denature phycocyanin (PC) and decrease its visible light absorption peak , fluorescence peak and degree of fluorescence polarization . However, allophycocyanin (APC) was insensitive to Cu~(2+). The results suggested that Cu~(2+) acts on PC of PBS and inhibits light absorption and transmission of PBS.

Key words: Spirulina maxima;Copper ion;Phycobiliprotein;Photosynthesis

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引用本文：

李建宏, 邰子厚, 曾昭琪. Cu~(2+)对蓝藻 Spirulina maxima光合作用的抑制机理[J]. 生理学报 1997; 49 (1): .

Li Jianhong, Tai Zihou, Zeng Zhaoqi. Mechanism of Cu~(2+) inhibiting photosynthesis of Spirulina maxima. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).