过刊浏览

活性氧在外源乙烯诱导内源乙烯产生过程中的作用

柯德森, 王爱国, 罗广华

中科院华南植物研究所.广东, 广州 510650

摘要

外源乙烯在一定的条件下明显抑制了超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性，提高了超氧化物阴离子自由基O~(.)_(2)和过氧化氢(H_(2)O_(2))的产率， 从而有效地诱导了内源乙烯产生的增加；外源O~(.)_(2)和H_(2)O_(2)对乙烯产生的促进作用及外源活性氧清除剂对乙烯产生的抑制作用也为此提供了证明。 乙烯对植物生理过程的调节机制之一就是通过影响活性氧清除酶活性，从而调节各种活性氧在体内的平衡。

关键词： 乙烯; SOD; CAT; 超氧阴离子自由基; 过氧化氢

The effect of activated oxygen during the production of endogenous ethylene induced by exogenous ethylene

Ke Deshen, Wang Aiguo, Luo Guanghua

South China Institute of Botany, The Chinese Academy of Sciences.　Guangzhou 510650, Guangdong

Abstract

After the treatment with various concentrations of ethrel for　different time, the rate of production of ethylene in etiolated　seedlings of Phaseolus radiatus L., the activity of SOD and CAT,and the　rate of production of O~(.)_(2) and H_(2)O_(2) were measured.Under some conditions, exogenous ethylene decreased the activity of　superoxide dismutase (SOD) and catalase (CAT), increased the rate of　production of superoxide radical O~(.)_(2) and H_(2)O_(2), and induced effectively the increase of the evolution of endogenous ethylene. There is a　threshold value for each of the above effects of exogenous ethylene: it　was 10~(-2)　#mu#mol/L and 10_(-1) #mu#mol/L for the inhibition of SOD and CAT respectively, and was 10_(-2) #mu#mol/L for the production of active oxygen and of endogenous ethylene.On the other hand, endogenous ethylene production was increased by　adding exogenous O~(.)_(2) (Na_(-2)S_(-2)O_(-3)) and H_(2)O_(2). The scavenger of O~(.)_(2)(SOD), and the　scavenger of H_(2)O_(2) (CAT) inhibited effectively the production of　endogenous ethylene(Fig.7). Exogenous ethylene can induce effectively　the production of endogenous ethylene.Because the involvement of　O~(.)_(2)) and H_(2)O_(2) in ethylene biosynthesis has been identified, the production of endogenous ethylene induced by exogenous ethylene is caused possibly by an increase of the rate of production of O~(.)_(2) and H_(2)O_(2), and by a decrease of the activity of the SOD and CAT. This may be the mechanism of induction of production of endogenous ethylene by exogenous ethylene.

Key words: Ethylene;SOD;CAT;Superoxide radical;H_(2)O_(2)

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

柯德森, 王爱国, 罗广华. 活性氧在外源乙烯诱导内源乙烯产生过程中的作用[J]. 生理学报 1997; 49 (1): .

Ke Deshen, Wang Aiguo, Luo Guanghua. The effect of activated oxygen during the production of endogenous ethylene induced by exogenous ethylene. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).