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渗调物质和fluridone对离体花生胚蛋白质合成的调节

林鹿, 傅家瑞

华南理工大学轻工食品学院.广东,广州 510641；中山大学生物系.广东,广州 510275

摘要

离体花生胚发育过程中,渗透调节物质提高了胚内源ABA含量,促进了贮藏蛋白质花生球蛋白和伴花生球蛋白II两个组分的合成,诱导和促进LEA蛋白的合成,内源ABA合成抑制剂fluridone抑制了渗调物质的作用。

关键词： 渗调物质; Fluridone; 贮藏蛋白; LEA蛋白

Effect of osmoticum and fluridone on synthesis of storage proteins and LEAproteins of peanut embryos cultured in vitro

Lin Lu, Fu Jiarui

Faculty of Light Industry and Food Chemistry Engineering, South China University of Technology. Guangzhou 510641, Guangdong

Abstract

Results of the experiment showed that mannitol as an osmoticum raised the endogenous ABAcontent of peanut (Arachis hypogaea L.) embryos cultured in vitro. The curve of the change of endogenous ABA content was similar to that of the speed of synthesis of arachin and coarachin of storage proteins, and ABA also induced and promoted the synthesis of LEA protein in peanut embryos cultured in vitro. Fluridone, an inhibitor of endogenous ABAsynthesis, decreased the content of endogenous ABAin peanut embryos cultured in vitro, its effect on the development of peanut embryos in vitro was opposite to that of the mannitol osmoticum. It issuggested that the effect of mannitol on the development of peanut embryos is through the action of endogenous ABA, but the mechanisms by which they regulate the germination of peanut embryos are different.

Key words: Osmoticum;Fluridone;Storage proteins;LEA proteins

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引用本文：

林鹿, 傅家瑞. 渗调物质和fluridone对离体花生胚蛋白质合成的调节[J]. 生理学报 1996; 48 (4): .

Lin Lu, Fu Jiarui. Effect of osmoticum and fluridone on synthesis of storage proteins and LEAproteins of peanut embryos cultured in vitro. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).