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表油菜素内酯对拟南芥细胞分化的影响

陈季楚, 徐玛娣, 赵毓橘

中科院上海植物生理研究所.上海 200032

摘要

研究了表油菜素内酯(epi-BR)对拟南芥细胞体外分化的影响。表明epi-BR不仅能促进愈伤组织的增殖,而且还能有效地诱导愈伤组织转绿,继而分化绿芽和长成小植株,其诱导频率高达70%以上。电镜观察表明,epi-BR诱导的转绿细胞中的叶绿体发育正常。

关键词： 表油菜素内酯; 拟南芥; 愈伤组织; 细胞分化

Effects of epibrassinolide on cell differentiation in Arabidopsis thaliana

Chen Jichu, Xu Madi, Zhao Yuju

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences. Shanghai 200032

Abstract

Arabidopsis thaliana have been used to investigate the role of epibrassinolide (epi-BR) in cell differentiation and regeneration in vitro. The results show that calli cultured on MS medium supplemented with 0.05, 0.5 or 5.0mg/L epi-BR formed much green buds and shoots. Supplementing the culture medium with epi-BR and 0.1mg/L KT induced the greening of callus and bud formation, but KT did not induce this differentiation alone. In addition, electron microscopic examination shows that normal chloroplasts were contained in cell of green callus cultured on medium with epi-BR.

Key words: Epibrassinolide (epi-BR);Arabidopsis thaliana;Callus;Cell differentiation

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引用本文：

陈季楚, 徐玛娣, 赵毓橘. 表油菜素内酯对拟南芥细胞分化的影响[J]. 生理学报 1996; 48 (4): .

Chen Jichu, Xu Madi, Zhao Yuju. Effects of epibrassinolide on cell differentiation in Arabidopsis thaliana. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).