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叶绿体atpE基因表达产物的生化特性

史瑾, 魏家绵, 沈允钢

中科院上海植物生理研究所.上海 200032

摘要

在细菌中表达的叶绿体atpE基因产物#epsilon#亚基蛋白对不同方式激活的叶绿体ATPase均有抑制作用,而其抗血清则促进ATPase活力。E. coli中表达的#epsilon#亚基蛋白在光合磷酸化反应中对循环和非循环光合磷酸化都有促进作用,其抗血清对循环光合磷酸化有抑制作用,而对非循环光合磷酸化则起促进作用。

关键词： 叶绿体; atpE基因; ε亚基; ATPase; 光合磷酸化

The properties of expressed product of chloroplast atpE gene

Shi Jin, Wei Jiamian, Shen Yungang

Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032

Abstract

The maize chloroplast atpE gene, which encodes the #epsilon# subunit of CF_(1), the coupling factor, was over-expressed in E. coli NM522 and then the expressed product was purified. The purified product was found to inhibit the ATPase activity of CF_(1) activated by heat or methanol. The antiserum raised against the expressed product, stimulated ATPast activity, and also decreased the quenching of 9-aminoacridine fluorescence. This result can be explained by the antiserum specific binding of the #epsilon# subunit from the CF_(1), which alleviated the function of #epsilon# subunit. The expressed product stimulated cyclic and non-cyclic photophosphorylation and its antiserum stimulated non-cyclic photophosphorylation but inhibited cyclic photophosphorylation, which can be explained by assuming that the two types of photophosphorylation utilize differently situated CF_(1) in thylakoids. Perhaps, ATP formation by non-cyclic photophosphorylation is only synthesized by CF_(1) of unappressed part of grana thylakoids, but that by cyclic photophosphorylation is pressed part of grana thylakoids but also CF_(1) of stroma thylakoids.

Key words: Chloroplast;AtpE gene;ε subunit;ATPase;Photophosphorylation

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引用本文：

史瑾, 魏家绵, 沈允钢. 叶绿体atpE基因表达产物的生化特性[J]. 生理学报 1996; 48 (3): .

Shi Jin, Wei Jiamian, Shen Yungang. The properties of expressed product of chloroplast atpE gene. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).