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光和底物对乙醇酸氧化酶的基因表达的诱导

徐杰, 李明启, 施教耐

华南农业大学生物系光合作用研究室.广东,广州 510642；中科院上海植物生理研究所.上海 200032

摘要

从菠菜中提纯了乙醇酸氧化酶并制备其抗体,经免疫双扩散、Western blot和Northern blot证实水稻和豌豆黄化苗中不存在乙醇酸氧化酶。在黑暗中,底物可促进该酶基因的表达,而在黄化苗光照初期,推测光可能是不经过底物促进该酶基因的表达。

关键词： 乙醇酸氧化酶; 基因表达; 光; 底物

Gene expression of glycolate oxidase induced by light and substrate glycolic acid

Xu Jie, Li Mingqi, Shi Jiaonai

Laboratory of Photosynthesis Research,Department of Agricultural Biology,South China Agricultural University.Guangzhou 510642；China；Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032

Abstract

Glycolate oxidase was purified from green leaves of spinach and its antibody was prepared. By Western blot, immunity double-diffusion and Northern blot, we verified that glycolate oxidase did not exist in etiolated rice seedling; but the gene expression of glycolate oxidase could be induced either by glycolate or by light alone.

Key words: Glycolate oxidase;Gene expression;Light;Substrate

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引用本文：

徐杰, 李明启, 施教耐. 光和底物对乙醇酸氧化酶的基因表达的诱导[J]. 生理学报 1996; 48 (3): .

Xu Jie, Li Mingqi, Shi Jiaonai. Gene expression of glycolate oxidase induced by light and substrate glycolic acid. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).