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Verticillium albo-atrum激发子诱导的番茄组织培养细胞的反应性氧迸发

刘曼西, Kolattukudy P E

华中理工大学生物工程系.湖北,武汉 430074；Biotechnology Center, The Ohio State University.Columbus,OH 43210

摘要

从对Verticillium albo-atrum感抗性不同的两株近等位基因蕃茄获得细胞悬浮培养物S-GCR26和R-GCR218。用V.albo-atrum激发子处理后,敏感性的S-GCR26未表现出明显的反应性氧改变。抗性的R-GCR218则响应以应性氧迸发。反应性氧迸发中的细胞,其反应性氧累积量与激发子浓度的关系呈近S形。悬浮介质、某些无机离子、培养物的生长状况和培养日龄等因素均影响细胞对激发子的反应性氧迸发响应。

关键词： 番茄细胞; 激发子; 反应性氧迸发

Oxidative burst in tomato cell culture induced by the elicitor from Verticillium albo-atrum

Liu Manxi, Kolattukudy P E

Huazhong University of Science and Technology.Wuhan 430074,Hubei

Abstract

The oxidative burst in two near isogenic tomato cell cutures induced by the elicitor from V.albo-atrum was investigated. The oxidative bursts of resistant and suceptible cell lines in Gamborg^s B_(5)/2% sucrose or in 2% sucrose only were compared Resistant cells showed a much higher level of reactive oxygen 20 min after the elicitor was added in two media.Cell age growth density,shaking,concentration of the elicitor,some oxidase and cathions etc. affected the peak value of oxidative burst in resistant tomato cell cutures. However, the pattern of the time course of oxidative burst was the same.The reactive oxygen species were increased rapidly at about 10 min and up to its peak at 20 min.There was always a difference in oxidative burst between the two near isogenic tomato cell tultures and the relative light units in resistant cell culture exhibited by luminol chemiluminscence was 1000 to 1000 times that in the susceptible one.The dependence of oxidative burst on concentration can be expressed by a sigmoid curve.

Key words: Tomato cells;Elicitor;Oxidative burst

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引用本文：

刘曼西, Kolattukudy P E. Verticillium albo-atrum激发子诱导的番茄组织培养细胞的反应性氧迸发[J]. 生理学报 1996; 48 (2): .

Liu Manxi, Kolattukudy P E. Oxidative burst in tomato cell culture induced by the elicitor from Verticillium albo-atrum. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).