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抗旱性不同的两个大豆品种对外源H_(2)O_(2)的响应

许长成, 邹琦, 樊继莲, 赵世杰, 孟庆伟

山东农业大学植物生理生化研究室.山东,泰安 271018

摘要

两个品种的大豆叶圆片经10~(-4)mol/L和10~(-3)mol/L的H_(2)O_(2)处理12h后,超氧物歧化酶(SOD)、过氧化氢酶(CAT)与谷胱甘肽还原酶(GR)活性明显增加,但10~(-2)mol/L的H_(2)O_(2)处理却使这些酶活性降低。抗旱性较强的大豆品种小粒豆1号较抗旱性较弱的鲁豆4号能维持较高的叶绿素含量和较高的SOD、CAT及GR活性,对H_(2)O_(2)的抗性较强。50#mu#mol/L的亚胺环乙酮(CHM)能消除H_(2)O_(2)对SOD、CAT与GR活性的刺激作用,而同样浓度的放线菌素D(AMD)则不能。

关键词： 外源H_(2)O_(2); 超氧物歧化酶; 过氧化氢酶; 谷胱甘肽还原酶; 抗旱性; 大豆

Response of two soybean cultivars with different drought resistance to exogenous H_(2)O_(2)

Xu Changcheng, Zou Qi, Fan Jilian, Zhao Shijie, Meng Qingwei

Plant Physiology and Biochemistry Laboratory,Shandong Agricultural University.Taian 271018,Shandong

Abstract

Leaf discs of a small-seed soybean cultivar Xiaolidou No. 1 (drought-resistant) and a commonly cultivated cultivar Ludou No. 4(drought-sensitive) were incubated in solutions containing different concentrations of H_(2)O_(2) for 12 h in light and in darkness.The activities of enzymes involved in the scavenging of active oxygen species were analyzed. Measurement of chlorophy11 contents in leaf discs showed a stronger resistance of Xiaolidou No. 1 than Ludou No. 4 to H_(2)O_(2) both in light and in darkness. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were increased by sublethal concentrations of H_(2)O_(2) and were higher in Xiaolidou No. 1 than in Ludou No. 4. This increase in the activities of SOD,CAT and GR was inhibited by the protein synthesis inhibitor cycloheximide (CHM),but not by actinomycin (AMD). At higher concentrations of H_(2)O_(2) the activities of all these enzymes decreased, especially in Ludou No. 4. These results suggest that the induction of the activities of SOD,Cat and Rr in response to sublethal concentration of H_(2)O_(2) involves denovo protein synthesis, probably at the level of translation by long-life mRNA.

Key words: Exogenous hydrogen peroxide;Superoxide dismutase;Catalase;Glutathione reductase;Drought resistance;Soybean

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引用本文：

许长成, 邹琦, 樊继莲, 赵世杰, 孟庆伟. 抗旱性不同的两个大豆品种对外源H_(2)O_(2)的响应[J]. 生理学报 1996; 48 (1): .

Xu Changcheng, Zou Qi, Fan Jilian, Zhao Shijie, Meng Qingwei. Response of two soybean cultivars with different drought resistance to exogenous H_(2)O_(2). Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).