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用GC-MS检测苹果种子层积过程中内源MeJA、GA_(3)、GA_(4)和GA_(7)的变化

马焕晋, Blake P S, Browning G

中国农业科学院果树研究所.辽宁,兴城 125100；园艺研究国际.East Malling

摘要

苹果种子层积40d后,萌发率及胚轴鲜重随着层积日数的增加而迅速增加。层积50d时,GA_(3)和GA_(7)含量分别达到高峰,在子叶中以GA_(3)为主,在胚轴中则以GA_(7)含量最高。层积对GA_(4)的含量变化影响不大。表明不同种类GA对低温处理的反应同不,发生器官和作用部位也有区别。MeJA在5℃和17℃条件下其含量变化无明显区别,但受温度的影响较大。

关键词： 苹果种子; 层积; 低温; GAs; MeJA

Changes in content of endogenous methyl jasmonate and gibberellins A_(3),A_(4) and A_(7) measured by gas chromtograph-mass spectrometry during stratification of apple Malus pumila l. seeds

Ma Huanpu, Blake P S, Browing G

Institute of Pomology,The Chinese Academy of Agricultural Sciences. Xingcheng 125100,Liaoning；UK

Abstract

Endogenous GA_(1),GA_(3),GA_(4)GA_(7) and methyl jasmonate (MeJA) were measured by GC-MS in cotyledon and embryonic axis of aplle seeds at each stage of stratification (5℃) and warm moist storage(17℃).The results showed that the percentage of seed germination and fresh weight of axes increased with time after 40 d of stratification. The contents of GA_(3) and GA_(7) also increased markedly at low temperature, a peak of GA_(3) was seen in the cotyledon and one of GA_(7) in the axis on 50th d of stratification No GA_(1) was detected at all stages of stratification, and no significant effect of low temperature on the level of GA_(4) was found in both cotyledon and axis. The pattern of MeJA change was not temperature-dependent but was evidently affected by high moisture. It can be suggested that GA_(3) and GA_(7) are involved in the release of dormancy by low temperature and there might be differences between GA_(3) and GA_(7).

Key words: Apple seed;Stratification;Low temperature;Gibberellins;Methyl jasmonate

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引用本文：

马焕晋, Blake P S, Browning G. 用GC-MS检测苹果种子层积过程中内源MeJA、GA_(3)、GA_(4)和GA_(7)的变化[J]. 生理学报 1996; 48 (1): .

Ma Huanpu, Blake P S, Browing G. Changes in content of endogenous methyl jasmonate and gibberellins A_(3),A_(4) and A_(7) measured by gas chromtograph-mass spectrometry during stratification of apple Malus pumila l. seeds. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).