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温度对温敏核雄性不育水稻花粉育性与花药蛋白质的影响

黄庆榴, 唐锡华, 茅剑蕾

中科院上海植物生理研究所,上海 200032

摘要

温度对温敏不育水稻二九青S花粉育性的影响极为明显,在30℃高温下,其花粉接近全不育;20℃低温下花粉不育度亦高,但不及30℃高温下显著。与原种二九青相比,高低温均使二九青S花药可溶性蛋白质含量明显增加,低温下更为显著;蛋白质SDS-PAGE图谱表明经30℃处理的二九青S花药蛋白质组分比原种二九青少了3条谱带。安农S-1在高温下花药中无花粉发育;而花药可溶性蛋白质含量高于适温(25℃)下;SDS-PAGE蛋白质图谱也表明高温下少1条谱带。

关键词： 温敏核雄性不育水稻; 温度; 花粉育性; 花药蛋白质

Effects of temperature on pollen fertility and anther protein in thermo-sensitive genic male sterile rice

Huang Qingliu, Tang Xihua, Mao Jianlei

Shanghai Insititute of Plant Physiology, The Chinese Academy of Sciences.Shanghai 200032

Abstract

The effects of temperature on pollen fertility as well as on the content and components of anther protein in "thermo-sensitive genic male stertile rice"(TGMSR) Er-jiu-Qing S and An-Nong S-1 were investigated. Temperature has very significant effects on pollen fertility and anther protein. Under 30℃, most of pollen grains in Er-jiu-Qing S were aborted. Under 20℃, the percentage of fertile pollen grains in Er-Jiu-Qing S was also very low.In comparison with 25℃, soluble protein content of anther in Er-Jiu-Qing S under 30℃and 20℃ was remarkably higher. The anther protein components in Er-jiu-Qing S differ significantly from its ancient line Er-Jiu-Qing under 30℃.In SDS-PAGE pattern, protein bands with MW 13, 13.5 and 23.5 kD were absent in Er-Jiu-Qing S.Under 30℃, all pollen grains of An-Nong S-1 were aborted and soluble protein content of anther higher than 25℃ treatment. Anther protein components under 30℃ are differed from those under 25℃. In SDS-PAGE pattern of anther protein of 30℃ treatment, the protein band with MW 12 kD was absent. These results indicate that the content and components of the anther protein are related to pollen fertility in indica "TGMSR" as well as in japonica "PGMSR".

Key words: Thermo-sensitive genic male aterile rice;Temperature;Pollen fertility;Anther protein

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引用本文：

黄庆榴, 唐锡华, 茅剑蕾. 温度对温敏核雄性不育水稻花粉育性与花药蛋白质的影响[J]. 生理学报 1996; 48 (1): .

Huang Qingliu, Tang Xihua, Mao Jianlei. Effects of temperature on pollen fertility and anther protein in thermo-sensitive genic male sterile rice. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).