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米曲霉中的紫草色素诱导物对滇紫草细胞代谢的影响

宁文, 郁taotao, 曹日强

南京大学生物科学与技术系.江苏,南京 210093

摘要

在滇紫草细胞培养d8时加入来源于米曲霉的紫草色素诱导物,12h后滇紫草细胞中紫草色素含量增加,其衍生物之一的乙酰紫草素的相对含量增加,胞内可溶蛋白合成量上升,细胞对碳源消耗增多,而细胞生长受到抑制。处理12h后,培养液电导率下降,pH上升,K~(+)外泄和Na~(+)大量内流,表明离子跨膜运输发生改变。

关键词： 米曲霉; 诱导物; 滇紫草; 代谢变化

Effect of an shikonin elicitor produced by Aspergillus oryzae on the metabolism of Onosma paniculatum cells

Ning Wen, Yu Taotao, Cao Riqiang

Department of Biology Science and Technology, Nanjing University.Nanjing 210093,Jiangsu

Abstract

An shikonin elicitor produced by Aspergillus could affect the metabolism of Onosma cells. 12 h after the elicitor was added on the 8th day, the shikonin derivatives content, the relative content of one of derivatives-acetylshikonin,the formation of intracellular soluble protein and the consumption of sucrose increased, but cell growth was inhibited permanently. 12h after elicited treatment, the medium conductivity decreased,medium pH value increased,K~(+) flowed out and Na~(+) flowed in, showed anion transport change over membrane caused by the elicitor. At the same time the concentration of Ca~(2+) in medium increased quickly.

Key words: Aspergillus oryzae;Elicitor;Onosma Paniculatum;Metabolic changes

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引用本文：

宁文, 郁taotao, 曹日强. 米曲霉中的紫草色素诱导物对滇紫草细胞代谢的影响[J]. 生理学报 1996; 48 (1): .

Ning Wen, Yu Taotao, Cao Riqiang. Effect of an shikonin elicitor produced by Aspergillus oryzae on the metabolism of Onosma paniculatum cells. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).