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增溶抽提#beta#-银环蛇毒素结合蛋白及某些突触前毒素对其结合的抑制作用

沈国光, 卓晓亮, 张新妹, 徐科

中科院上海生理研究所. 上海 200031

摘要

#beta#-银环蛇毒素结合蛋白抽提液的比结合活性为200-400fmol/mg蛋白, 比膜制备物的比结合活力提高一倍。抽提得率为50％-70％。抽提液与~(125)I标记的#beta#-银环蛇毒素的结合可被曼巴蛇神经毒素完全抑制, 其IC_(50)约8×10~(-8)mol/L。但另一种#beta#型神经毒素--#beta#-蝮蛇神经毒素却完全不能抑制这种结合。

关键词： β－银环蛇神经毒素; β－银环蛇毒素结合蛋白; β－蝮蛇神经毒素; 曼巴蛇神经毒素; 大鼠; 膈肌

Solubilization of #beta#-bungarotoxin-binding protein from rat diaphragm and inhibition of its binding by some other presynaptic neurotoxins

Shen Guoguang, Zhuo Xiaoliang, Zhang Xinmei, Xu Ke

Shanghai Institute of Physiology, Chinese Academy of Sciences. Shanghai 200031

Abstract

This paper reports the solubilization of #beta#-bungarotoxin- binding protein from rat diaphragm membrane preparations with Triton X-100. The specific binding activities of the detergent extracts are 200-400 fmol/mg of protein, and the yields are about 50%-70%. The binding of ~(125)I-#beta#-bungarotoxin to the extracts could be completely inhibited by dendrotoxin, with an IC_(50) of about 8×10~(-8) mol/L. Another #beta#-agkistrodotoxin, however, could not inhibit the ~(125)I-#beta#-bungarotoxin binding at all. This indicates that the acting sites of #beta#-agkistrodotoxin on the presynaptic membranes are different from those of #beta#-bungarotoxin.

Key words: #beta#-bungarotoxin;#beta#-bungarotoxin-binding protein #beta#-agkistrodotoxin;Dendrotoxin;Ratdiaphragm;;

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引用本文：

沈国光, 卓晓亮, 张新妹, 徐科. 增溶抽提#beta#-银环蛇毒素结合蛋白及某些突触前毒素对其结合的抑制作用[J]. 生理学报 1996; 48 (1): .

Shen Guoguang, Zhuo Xiaoliang, Zhang Xinmei, Xu Ke. Solubilization of #beta#-bungarotoxin-binding protein from rat diaphragm and inhibition of its binding by some other presynaptic neurotoxins. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).