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AF64A对大鼠隔复合体内一氧化氮合酶和乙酰胆碱共存神经元的影响

余福林, 单红英, 董新文

中科院上海生理研究所. 上海 200031

摘要

AF64A对大鼠隔复合 体内不同细胞亚群内含一氧化氮合酶(NOS)或不含NOS的两种胆碱能神经元的毒害作用不尽相同, NADPH-对黄递酶与胆碱乙酰基转移酶双标记的胆碱能神经元不因为有NOS的共存而抵御AF64A的神经毒害作用, 中间亚群内含NOS与不含NOS的胆碱能神经元可能具有不同的纤维投射。

关键词： AF64A; 一氧化氮合酶; NADPH－黄递酶组织化学; 胆碱乙酰基转移酶; 免疫细胞化学; 隔复合体

Effects of AF64A on neurons containing both nitric oxide synthase and choline acetyltransferase in the rat septal complex

Yu Fulin, Shan Hongying, Dong Xinwen

Shanghai Institute of Physiology, Chinese Academy of Sciences. Shanghai 200031

Abstract

Ethylcholine mustard aziridinium ion(AF64A), a neurotoxic choline analog, was injected(ICV)bilaterally (1.5nmol/ventricle, n=10)into male adult rats to induce a model of Alzheimer^s disease(AD). One month later, using NADPH-diaphorase (NADPH-d) histochemistry followed by choline acetyltransferase(ChAT) immunocytochemistry(PAP)on the coronal sections of the septal complex, double-staining experiments were performed to assay the alterations of septalcholinergic neurons coexisted with nitric oxide synthase (NOS). These results indicate that: (1) the administration of AF64A has different effects on the cholinergic neurons with or without NOS in different subgroups of the septal complex,and the NADPH-d+ChAT double labelled neurons resist the neurotoxicity of AF64A; (2) in the intermediate subgroup, the cholinergic neurons containing NOS may have projections different from those without NOS.

Key words: AF64A;Nitric oxide pethase;NADPH-diaphorase histochemistry;Chorine acetylferase;Immunocpehemistry;Septal complex

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引用本文：

余福林, 单红英, 董新文. AF64A对大鼠隔复合体内一氧化氮合酶和乙酰胆碱共存神经元的影响[J]. 生理学报 1996; 48 (1): .

Yu Fulin, Shan Hongying, Dong Xinwen. Effects of AF64A on neurons containing both nitric oxide synthase and choline acetyltransferase in the rat septal complex. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).