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电针引起脊髓P物质释放的频率依赖性

沈上, 边景檀, 田津斌, 韩济生

北京医科大学神经科学研究中心. 北京 100083

摘要

观察P物质(SP)释放的频率依赖性, 电针频率选择2、4、8、15、30和100Hz, 分别收集电针期间及电针前后各30min的脊髓灌流液, 通过放射免疫方法测定大鼠电针有效组和电针无效组P物质免疫活性。结果表明对于电针有效组, 刺激引起SP释放有频率依赖性; 对于电针无效组, 不论应用何种频率, 电针前后脊髓灌流液中SP-ir变化不大。

关键词： P物质; 电针镇痛; 放射免疫分析; 脊髓

Frequency dependence of substance P release by electroacupuncture in rat spinal cord

Shen Shang, Bian Jingtan, Tian Jinbin, Han Jisheng

Neuroscience Research Center, Beijing Medical University. Beijing 100083

Abstract

In the present study we observed the frequency dependence of substance P (SP) release from rat spinal cord, with the frequencies of EA set at 2, 4, 8, 15, 30 and 100 Hz, The spinal perfusate was collected in 3 periods of 30 min before, during and after EA, and the immunoreactive SP (SP-ir)was measured by radioimmunoassay (RIA). The effectiveness of EA-induced analgesia was assessed by tail flick latency (TFL). Rats showing an increase of TFL over 40% was considered as EA responder. The results showed that in the responders, SP-ir in spinal perfusate showed a moderate decrease during 2 Hz EA, (P<0.01 compared with baseline level), no change in the 4 Hz EA group, and a marked increase during 8, 15, 30 and 100 Hz EA (P<0.01), with maximal increase occurring at 15 Hz(P<0.001).

Key words: Subonce P;Electroacupuncture;Radioimmunoassay;Spinal cord

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引用本文：

沈上, 边景檀, 田津斌, 韩济生. 电针引起脊髓P物质释放的频率依赖性[J]. 生理学报 1996; 48 (1): .

Shen Shang, Bian Jingtan, Tian Jinbin, Han Jisheng. Frequency dependence of substance P release by electroacupuncture in rat spinal cord. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).