过刊浏览

GM-CSF和IL-3对脐带血CD34~(+)细胞植入骨髓基质的促进作用

裴雪涛, 吴祖泽, Coutinho L H, Testa N G, Dexter T M

军事医学科学院放射医学研究所. 北京 100850；英国Paterson肿瘤研究所. 英国

摘要

经2h铺展贴壁后, 对照组只有36％的CD34~(+)细胞植入基质, 而生长因子预处理 组则有68-89.6％的CD34~(+)细胞植入基质。在长期液体培养体系中则显示了植入CD34~(+)细胞多的处理组造血重建快速而持久。表明GM-CSF和IL-3预处理将明显提高脐带血移植效率。

关键词： 脐带血; CD34~(＋)造血祖细胞; 生长因子; 移植效率

The effect of GM-CSF/IL-3 on the grafting efficiency of CD34~(+) hemopoietic progenitor cells from unbilical cord blood

Pei Xuetao, Wu Zuze, Coutinho L H, Testa N G, Dexter T M

Beijing Institute of Radiation Medicine, Chinese Academy of Military Medicine. Beijing 100850

Abstract

CD34~(+) cells were isolated from human umbilical cord blood by using a high-gradient magnetic cell sorting system with anti-CD34 monoclonal antibody coated with microbeads, and then inoculated noto a pre-formed irradiated bone marrow stroma to evaluate the grafting efficiency of CD34~(+) cells with rhGM-CSF or IL-3, alone or in combination. The results showed that only 36% CD34~(+) cells in the control, and 68-89.6% in the groups with growth factors treatment seeded to the stroma. A short incubation of CD34~(+) cells with growth factors could rapidly reconstitute a long-term hemopoiesis in the long-term liquid culture system. This demonstrates that a brief treatment of CD34~(+) cells with GM-CSF or IL-3 can improve the grafting efficiency of transplanted umbilical cord blood cells.

Key words: Umbilical cord blood;CD34~(+) hematopoietic progenitor cells;Growth factors; Grafting efficiency

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

裴雪涛, 吴祖泽, Coutinho L H, Testa N G, Dexter T M. GM-CSF和IL-3对脐带血CD34~(+)细胞植入骨髓基质的促进作用[J]. 生理学报 1995; 47 (5): .

Pei Xuetao, Wu Zuze, Coutinho L H, Testa N G, Dexter T M. The effect of GM-CSF/IL-3 on the grafting efficiency of CD34~(+) hemopoietic progenitor cells from unbilical cord blood. Acta Physiol Sin 1995; 47 (5): (in Chinese with English abstract).