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缺氧促进热休克蛋白70在肺动脉平滑肌细胞中的表达

冯晓东, 蔡英年

医科院基础医学研究所. 北京 100005

摘要

缺氧性肺动脉高压中肺动脉结构重组, 中膜平滑肌细胞增殖并迁移, 但机制不明。该 研究观察缺氧对肺动脉平滑肌细胞(PASMC)细胞周期、DNA合成及细胞增殖的影响, 并通 过观察缺氧对PASMC热休克蛋白70(HSP70)表达的影响, 初步探讨缺氧的作用是如何介导 的。结果表明缺氧可直接或协同内皮素-1促进PASMC DNA合成及细胞增殖, 并可增加HSP70在PASMC中的表达。

关键词： 缺氧; 肺动脉平滑肌细胞; 增殖; 热休克蛋白70

Hypoxia stimulates the expression of HSP70 in pulmonary artery smooth muscle cells

Feng Xiaodong, Cai Yingnian

Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. Beijing 100005

Abstract

Hypoxic pulmonary hypertension is characterized by remodeling of pulmonary artery, proliferation and migration of pulmonary artery smooth muscle cells (PASMC), the mechanism of which was studied by observing the efeect of hypoxia on DNA synthesis, eell cycle phase and transcription of heat shock protein 70 (HSP70). Our results indicate that hypoxia increase DNA synthesis, enhances the mitogenic effect of ET-1 on PASMC, stimulates quiescent PASMC entering S phase from G_(0)/G_(1) phase and up-regulates the mRNA level of HSP70. Heat shock proteins are conservative and important stress proteins rendering the cells more tolerable to dangerous stresses by promoting growth and survival of the cells.

Key words: Hypoxia;Pulmonary artery smooth muscle cells;Proliferation;Heat shock protein 70

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引用本文：

冯晓东, 蔡英年. 缺氧促进热休克蛋白70在肺动脉平滑肌细胞中的表达[J]. 生理学报 1995; 47 (5): .

Feng Xiaodong, Cai Yingnian. Hypoxia stimulates the expression of HSP70 in pulmonary artery smooth muscle cells. Acta Physiol Sin 1995; 47 (5): (in Chinese with English abstract).