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#alpha#_(1)肾上腺素受体亚型在家兔胸主动脉平滑肌细胞生长中的作用

黎洋, 韩启德

北京医科大学第三临床医学院. 北京 100083

摘要

去甲肾上腺素激动#alpha#_(1)-AR能促进平滑肌细胞DNA合成, 并有以下特点:#alpha#_(1A)激动促进平滑肌细胞DNA合成, 但#alpha#_(1B)激动抑制这一合成作用, #alpha#_(1A)促进DNA合成的作用与c-fos基因表达有关; #alpha#_(1)-AR的促生长作用与神经肽Y有协同作用, 而与血管紧张素Ⅱ仅有相加的作用; 培养的平滑肌细胞上#alpha#_(1B)有一定的固有活性, 表现为抑制平滑肌细胞DNA合成。

关键词： α_(1)肾上腺素受体; 受体亚型; 平滑肌细胞; 增殖; 血管

Effects of #alpha#_(1)-adrenergic receptor subtypes activation on proliferation of smooth muscle cells in rabbit aorta

Li Yang, Han Qide

The Third School of Clinical Medicine, Beijing Medical University. Beijing 100083

Abstract

The effect of #alpha#_(1)-Adrenoceptor (#alpha_(1)-AR) and its subtypes, #alpha#_(1A) and #alpha#_(1B), activation on the proliferation of cultured vascular smooth muscle cells (VSMC) was studied by [~(3}H]-thymidine (~(3)H-TdR) incorporation and in situ hybridization. Norepinephrine (NE) promotes DNA synthesis of VSMC. The action was induced by activation of #alpha#_(1A)-AR subtype and increase of c-fos gene expression. However, the DNA synthesis was inhibited by activation of #alpha#_(1B)-AR subtype. There was a synergetic effect on VSMC DNA synthesis between NE and neuropeptide Y (NPY). But only additional effect was found between NE and Ang II. More over, it was fonund that #alpha#&&(1B)-AR subtype showed significant constitutive inhibitory effect on VSMC DNA synthesis.

Key words: #alpha#_(1)-adrenoceptor;Receptor subtypes;Smooth muscle cell;Proliferation;Blood vessel

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引用本文：

黎洋, 韩启德. #alpha#_(1)肾上腺素受体亚型在家兔胸主动脉平滑肌细胞生长中的作用[J]. 生理学报 1995; 47 (5): .

Li Yang, Han Qide. Effects of #alpha#_(1)-adrenergic receptor subtypes activation on proliferation of smooth muscle cells in rabbit aorta. Acta Physiol Sin 1995; 47 (5): (in Chinese with English abstract).