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表油菜素内酯对黄瓜子叶过氧化物酶活性和可溶性蛋白含量的影响

丁文明, 赵毓橘

中科院上海植物生理研究所. 上海 200032

摘要

0.5mg/L的表油菜素内酯(epi-BR)能显著地促进黄瓜子叶叶绿素a、叶绿素b的含量和 叶绿素a/叶绿素b比值的下降, 表明epi-BR能促进子叶的衰老。从过氧化物酶(POD)的活性测定及同工酶谱发现epi-BR可提高其活性, 暗示它可能通过提高子叶POD的活性而加 速子叶叶绿素的降解。另一方面, epi-BR促进子叶可溶性蛋白含量的下降, 其中主要是RuBP羧化酶含量的下降, 同时, epi-BR引起子叶游离氨基酸的累积。

关键词： 表油菜素内酯; 黄瓜子叶; 过氧化物酶; 叶绿素; 可溶性蛋白; 游离氨基酸

Effect of epi-BR on activity of peroxidase and soluble protein content of cucumber cotyledon

Ding Wenming, Zhao Yuju

Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai 200032

Abstract

It is shown that epi-brassinolide (epi-BR, 0.5mg/L), a new synthetic brassinolide, significantly reduced the content of chlorophyll a, chlorophyll b in cucumber cotyledons and the ratio of chlorophyll a/ chlorophyll b. Further studies showed that in the cotyledons epi-Br increased the activity of peroxidase, which is involved in the breakdown of the chlorophyll. In addition, epi-BR de-creased the content of soluble protein in cucumber cotyledons (mainly the RuBPcase) and caused the accumulatin of free aminc acids. All of these effects of epi-BR on cucumber cotyledons indicates that epi-BR accelerates the senescence of cucumber cotyledons.

Key words: Epi-brassinolide;Cucumber cotyledons;Peroxidase;Chlorophyll;Soluble protein;Free amino acids

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引用本文：

丁文明, 赵毓橘. 表油菜素内酯对黄瓜子叶过氧化物酶活性和可溶性蛋白含量的影响[J]. 生理学报 1995; 47 (3): .

Ding Wenming, Zhao Yuju. Effect of epi-BR on activity of peroxidase and soluble protein content of cucumber cotyledon. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).