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外源酚对凤眼莲体内酚含量和与酚代谢有关酶的影响

汪敏, 郑师章

复旦大学环境与资源生物学系. 上海 200433

摘要

凤眼莲能够吸收和在体内聚集外源苯酚, 体内的酚含量随着环境中酚浓度的上升而上 升。从生长于含酚培养液中的凤眼莲体内能够检测到酚糖苷, 说明凤眼莲体内有酚糖苷 转移酶的存在。浓度小于50mg/L的外源酚能提高凤眼莲体内的多酚氧化酶和过氧化物酶 的活性。多酶氧化酶与过氧化物酶在线粒体和微粒体中均有不同程度的分布, 而酚糖苷 转移酶则不存在于这些细胞器中。

关键词： 凤眼莲; 多酚氧化酶; 过氧化物酶; 酚糖苷转移酶; 苯酚

Effects of exogenous phenol on phenol content and the enymes related to phenol metabolism in Eichhornia crassipes

Wang Min, Zheng Shizhang

Department of Environment and Resources Biology, Fudan University. Shanghai 200433

Abstract

E. crassipes) could absorb and concentrate exogenous phenolic compounds, and the content of phenol in the plant increased with its concentration in the medium. Phenol, concentrations at up to 50mg/L in the medium, increased the activities of polyphenol oxidase and peroxidase. The peroxidase of the plant did not hydroxylate phenol to catechol. Phenol glucosyltransferase was detected in E. crassipes) for the first time. The activities of the three enzymes studied were found to be different in mitochondria, microsomes and the remaining components (as supernatant, Quatitatively, phenol concentration in the medium had little effect on the activity of the phenol glucosyltransferase in the plant, but the method of its quantitative measurement needs further studies.

Key words: Eichhornia crassipes;Polyphenol oxidase;Peroxidase;Phenol glucosyltransferase;Phenol

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引用本文：

汪敏, 郑师章. 外源酚对凤眼莲体内酚含量和与酚代谢有关酶的影响[J]. 生理学报 1995; 47 (3): .

Wang Min, Zheng Shizhang. Effects of exogenous phenol on phenol content and the enymes related to phenol metabolism in Eichhornia crassipes. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).