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菠菜叶片中两种酶型的磷酸果糖激酶性质的比较

查静娟, 胡志元, 吴敏贤, 施教耐

中科院上海植物生理研究所. 上海 200032

摘要

从菠菜叶片中提取到两种依赖ATP的磷酸果糖激酶。其一分子量大于2000kD, 活力可被Pi、3-PGA、柠檬酸激活, 被PEP强烈抑制; 另一为小分子酶型, 分子量300kD, 其活性 受Pi、3-PGA、柠檬酸和PEP抑制。证明小分子酶型可能存在叶绿体中, 大分子酶型属于胞质酶。

关键词： 磷酸果糖激酶; 大分子酶型; 小分子酶型

Comparison between the properties of two forms of phosphofructokinase from spinach leaves

Zha Jingjuan, Hu Zhiyuan, Wu Minxian, Shi Jiaonai

Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Shanghai 200032

Abstract

Two forms of phosphofructokinase were separated by chromatography on a FPLC Sephacryl S-300 column from spinach leaf homogenate. The molecular weight of the large form was over 2000kD and that of the small form was 300kD. The large form was activated by inorganic phosphate, 3-PGA or citrate, but its activity was seriously inhibited by PEP. The inhibition could be relieved by Pi to some degree. The kinetic constants of the large form and the small form were: Mg~(2+) was required for the activity of both forms of PFK. The activity of large form was inhibited by a concentration of Mg~(2+) higher than 0.5mmol/L, while that of the small form was not affected.

Key words: ATP-dependent phosphofructokinase;Large form;Small form

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引用本文：

查静娟, 胡志元, 吴敏贤, 施教耐. 菠菜叶片中两种酶型的磷酸果糖激酶性质的比较[J]. 生理学报 1995; 47 (3): .

Zha Jingjuan, Hu Zhiyuan, Wu Minxian, Shi Jiaonai. Comparison between the properties of two forms of phosphofructokinase from spinach leaves. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).