过刊浏览

钙通道阻滞剂对肾上腺素能受体激动剂促血管平滑肌细胞增殖的抑制

李莹, 王睿, 林树新, 贾斌, 宋开梅

第四军医大学基础部. 陕西,西安 710032；唐都医院心脏内科. 陕西,西安 710032

摘要

观察到10~(-6)-10~(-5)mol/L去甲肾上腺素(NE)和10~(-7)-10~(-5)mol/L异丙基肾上腺素(ISO)可明显促进离体培养的血管平滑肌细胞(VSMC)的增殖和DNA的合成, 并呈剂量依赖效应, 该效应可为相应的受体阻断剂phentolamine (10~(-6)mol/L)和propranolol (10~(-5)mol/L)所抑制; nifedipine (10~(-6)mol/L)和verapamil (10~(-6)mol/L)分别与同样浓度的NE同时加入细胞培养液中, 其细胞计数和~(3)H-TdR掺入率分别较单用NE时显著降低(P<0.01), nifedipine与verapamil亦明显抑制ISO促VSMC增殖的作用。图6参6

关键词： 血管平滑肌细胞(VSMC); 去甲肾上腺素(NE); 异丙基肾上腺素(ISO); 肾上腺素能受体激动剂; 钙通道阻滞剂

Inhibitary effects of calcium channel antagonist on the proliferation of vascular smooth muscle cells induced by adrenoceptor activators

Li Ying, Wang Rui, Lin Shuxin, Jia Bin, Song Kaimei

Department of Pathophysiology, Fourth Military Medical University. Xi^an 710032,Shaanxi；China

Abstract

In culytured vascular smooth muscle cells (VSMC) of SD rat, it was demonstrated that 10~(-6)--10~(-5)mol/L NE and 10~(-7)---10~(-5)mol/L isoproterenol stimulated proliferation and ~(3)H--TdR incorporation of the cells in a dose dependent manner. These effets might be significantly inhibited by co--incubation with either #alpha#--receptor blocker phentolamine (10~(-6)mol/L) or #beta#--receptor blocker propranolol (10~(-50125mol/L). It was also found that cell counting and ~(3)H--TdR incorporation were markedly decreased after incubation nifedipine (10~(-6)mol/L) or verapamil (10~(-6)mol/L) with NE (P<0.01 as compared to that of NE group). It was further observed that nifedipine and verapamil inhibited DNA synthesis and proliferation of VSMC induced by isoproterenol.

Key words: Vascular smooth muscle cells;Norepinephrille;Isopyoterenol;Adrenoceptor activators;Calcium channel antagonist

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

李莹, 王睿, 林树新, 贾斌, 宋开梅. 钙通道阻滞剂对肾上腺素能受体激动剂促血管平滑肌细胞增殖的抑制[J]. 生理学报 1995; 47 (3): .

Li Ying, Wang Rui, Lin Shuxin, Jia Bin, Song Kaimei. Inhibitary effects of calcium channel antagonist on the proliferation of vascular smooth muscle cells induced by adrenoceptor activators. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).