过刊浏览

甘蔗幼叶原生质体分离过程中呼吸的变化及其影响因素

何若天, 覃伟

广西农业大学实验中心. 广西, 南宁 530005

摘要

甘蔗幼叶呼吸途径以EMP-TCAC为主, PPP活力不强; 游离原生质体EMP活力与幼叶组织相近, 但TCAC活力略有下降, PPP活力明显提高, 约为幼叶组织的两倍。甘蔗幼叶原生质体在用酶法分离过程中, 其组织呼吸速率开始时呈现短暂跃升后即迅速下降。导致下降的主因是细胞壁降解产物中的某些物质, 它们通过钝化呼吸途径中某些关键酶的活性而降低了呼吸活力。图6表3参19

关键词： 呼吸作用; 细胞壁降解酶; 毒性因素; 原生质体; 甘蔗

Change of respiration of young sugarcane leaf tissue during protoplasts isolation and some factors influencing them

He Ruotian, Qin Wei

Research Centre, Guangxi Agricultural University. Nanning 530005, Guangxi

Abstract

Following a transient rise at the early stage, the respiratory rate of the tissue from young sugarcane leaf declined quickly during the enzymatic isolation of protoplasts. In the presence of the mixture of cell wall degrading enzymes, the respiratory rate of the young leaf tissues decreased with an increase of the concentration of mannitol used as osmoticum, but to a lesser extent in the absence of the cell wall degrading enzymes, The respiratory rate decreased with an increase of cellulase concentration when the concentrations of pectinase and mannitol were kept constant in the mixture. As compared with the pectinase, the harmful effect of cellulase onthe respiration of young sugarcane tissues is more powerful. The respiration of freshly prepared protoplasts from young sugarcane leaf was inactivated by the cell wall degradation products (CWDP), water--extract of cell wall, active and denatural by boil cell wall degrading enzyme mixture, and the strongest inactivation was produced by CWDP which inactivated the activities of the key respiratory enzymes, suggesting that the decrease of respiratory activity might be caused mainly by the toxicity of CWDP during the enzymatic isolation

Key words: Respiration;Cell wall degrading enzyme;Toxic factor;Protoplast;Sugarcane

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

何若天, 覃伟. 甘蔗幼叶原生质体分离过程中呼吸的变化及其影响因素[J]. 生理学报 1995; 47 (1): .

He Ruotian, Qin Wei. Change of respiration of young sugarcane leaf tissue during protoplasts isolation and some factors influencing them. Acta Physiol Sin 1995; 47 (1): (in Chinese with English abstract).