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长时间低氧对大鼠颈动脉体培养细胞的细胞内pH和膜电位的影响

何淑舫

中科院上海脑研究所. 上海 200031

摘要

对实验组Sprague-Dawley(SD)大鼠,首次将其置于模拟5000m高度低氧环境的低压舱中饲养7-10d, 然后麻醉动物, 取出颈动脉体, 将其分离成单个细胞和细胞群体。这些细胞在低氧条件(11%O_(2), 5% CO_(2), 84% N_(2))下培养2-3d。取自正常SD大鼠的颈动脉体细胞被分为两组, 分别将其培养在常氧(21%O_(2), 5%CO_(2), 74%N_(2 ))或低氧环境中。球细胞的细胞内pH(pH_(i))和膜电位(MP)分别用H~(+)选择性微电级和常规微电极同时测量。发现长时间低氧降低球细胞 的pH_(i), 增加MP, 其变化程度远远大于急性低氧的影响, 而且当将细胞置于常氧中测量时其值不恢复。图3表1参15

关键词： 细胞内pH; 膜电位; 低氧; 颈动脉体; glomus细胞

Effects of long-term hypoxia of intracellular pH and membrane potential of cultured rat carotid body glomus cells

He Shufang

Shanghai Brain Research Institute, Chinese Academy of Sciences. Shanghai 200031

Abstract

Spraque-Dawley(SD) rats(weighing about 100 g) were kept in a decompression chamber for 7--10 days. Then the carotid bodies were removed from the anesthetized animals and dissociated to obtain individuals or small clusters of glomus cells. These cells were cultured under hypoxic condition(11% O_(2), 5% CO_(2), 84% N_(2))for 2--3days. Control samples from normal SD rats(normoxic rat)were cultured in normoxic(21% O_(2), 5% CO_(2), 74%N_(2))or hypoxic conditions. Intracellular pH(pHi) and membrane potential(MP)of glomus cells of both the groups were simultaneously measured with pH-sensitive and conventional microelectrodes. Long-term hypoxia decreased pHi and increased MP of the cultured glomus cells to a degree far greater than acute hypoxia did; The mean pHi and MP of the glomus cells of the normoxic rat, but cultured under hypoxic environment, recovered approximately to control values when measued under normoxic condition, whereas those of the long-term hypoxic rat cells did not.

Key words: Intracellular pH;membrane potential;Hypoxia;Carotid body;Glomus cell

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引用本文：

何淑舫. 长时间低氧对大鼠颈动脉体培养细胞的细胞内pH和膜电位的影响[J]. 生理学报 1995; 47 (3): .

He Shufang. Effects of long-term hypoxia of intracellular pH and membrane potential of cultured rat carotid body glomus cells. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).