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内皮衍生舒张因子对缺血(缺氧)、再灌注(复氧)心肌的保护作用

万梅, 于占久

河北省医学科学院病理生理研究室. 河北, 石家庄 050021

摘要

分别在大鼠Langendorff离体心脏灌流模型和培养的大鼠心肌细胞上观察了NO、NO的前体物 质L-精氨酸(L-Arg)、NO的合成阻断剂L-硝基精氨酸(L-NNA)对心肌缺血(缺氧)再灌注( 复氧)损伤的影响。结果: (1)离体心脏停灌30min后, 用含NO的K-H液再灌流时可显著增加 冠脉流量、左室内压及±dp/dt_(max), 而用含L-NNA的K-H液再灌流则作用相反。L-Arg可部分逆转L-NNA的收缩冠脉作用, 而其单独作用却不能改善心肌血供和心肌收缩力。NO降低心肌组织丙二醛(MDA)和N-乙酰-#beta#-D-氨基葡萄糖苷酶(NAG酶)含量, 而L-NNA则使之增加。(2)培养心肌细胞缺氧后复氧, 同时加入NO, L-Arg或L-NNA三种物质对心肌细胞内Ca~(2+)含量均无显著影响, 但均可降低缺氧再给氧培养液中MDA和NAG酶含量 。图2表4参12

关键词： 一氧化氮; 心肌缺血再灌注损伤; 心肌缺氧复氧损伤; Langendorff心脏; 心肌细胞; 氧自由基损伤

Protective effect of endothelium-derived relaxing factor on ischemic (hypoxic) and reperfused (reoxygenated) myocardium

Wan Mei, Yu Zhanjiu

Pathophysiology Laborotory, Hebei Academy of Medical Sciences. Shijiazhuang 050021, Hebei

Abstract

When heart perfusion was stopped for 30 min and reinstitued for 20 min with K-H buffer containing NO, the coronary flow rate(CFR), left ventricular pressure(LVP) and ±dp/dt_(max) increased significantly. When NO was replaced by L-NNA opposite effects were observed. L-Arg alone was without effect on CFR, LVP and ±dp/dt_(max), but attenuated the decreasing effect of L-NNA on CFR. NO decreased MDA and NAGase content of myocardium while L-NNA increased them. When cultured ventricular myocytes were subjected to hypoxia for 30 min and reozygenated for 20 min, none of the substances under investigation showed any effects on Ca~(2+)content of heart cells, but all of them decreased MDA, NAGase content of the culture tissue after reoxygenation. NO plays an important role in protecting myocardium from is chemic and reperfused injury by improving blood supply of reperfused myocardium and attenuation of oxygen free radical injury.

Key words: Nitric oxide;Myocardial ischemia and reperfusion injury;Myocardial hypoxia and re oxygenation injury;Langendorff heart;Myocyte;Oxygen free radical

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引用本文：

万梅, 于占久. 内皮衍生舒张因子对缺血(缺氧)、再灌注(复氧)心肌的保护作用[J]. 生理学报 1995; 47 (3): .

Wan Mei, Yu Zhanjiu. Protective effect of endothelium-derived relaxing factor on ischemic (hypoxic) and reperfused (reoxygenated) myocardium. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).