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兴奋性氨基酸介导的膜电流可被吗啡阻断

张敏, 聂琳, 刘磊, Wang Y T, Neuman R S, Bieger D

山东医科大学生理教研室. 山东, 济南 250012；纽芬兰纪念大学医学院. 加拿大

摘要

在Sprague-Dawiey大鼠的含有孤束核中央亚核及疑核神经元密集区的脑薄片上, 吗啡(3-5pmol)对疑核细胞内记录到的自发兴奋性突触后电位有抑制作用; 向灌流液内注入吗啡可使 电刺激孤束核在疑核记录到的兴奋性突触后电位的幅度降低71.1±6.2%(P<0.001), 纳洛酮(50nmol/L)可翻转这种抑制效应; 含有10#mu#mol/L吗啡的灌流液对NMDA(0.5-1pmol)、乙酰胆碱(3pmol)及Quisqualate(0.1-0.5pmol)引起的细胞膜去极化有不同程度的抑制作 用, 分别为38.1±5.7%(P<0.001), 32.8±5.5%(P<0.01)和29.6±7.1%(P<0.05)。图4参21

关键词： NMDA; 孤束核中央正核; 疑核神经元密集区; 吗啡; AMPA

Morphine blocked the exitatory amino acid mediated membrane current in ambigual motoneurons of the rat

Zhang Min, Nie Lin, Liu Lei, Wang Y T, Neuman R S, Bieger D

Department of Physiology, Shandong Medical University. Jinan 250012, Shandong

Abstract

Excitatory postsynaptic potentials(EPSPs) and responses of neurons in the compact formation of the neucleus ambiguus(AMBc) to pressure-ejected or bath-applied test substances were recorded intracellularly from sagittal slices of Sprague-Dawley rat medulla containing subnucleus centralis of solitary complex(NTSc), AMBc and solitarioambigual pathway. In 5 cells, recorded spontaneous EPSPs could be blocked by morphine (3--5pmol) to AMBc. Electrical stimulation of NTSc evoked EPSPs in AMBc neurons, the amplitude of which were decreased to 71.1±6.2%(P<0.001) with the addition to morphine. The morphine effect could be abolished by bath-applied naloxone (50nmol/L). The amplitude of membrane depolarization induced by pressure-ejected N-methyl-D-aspartate (NMDA, 0.5--1.0pmol), acetylcholine (3 pmol) and quisqualate(0.1--0.5pmol) to NTSc could also be decreased by bath-applied morphine(10 #mu#mol/L) respectively to 38.1±5.7%(P<0.001), 32.8±5.5%(P<0.01) and 29.6±7.1%(P<0.05).

Key words: NMDA;AMPA;Compact formation of the neucleus ambiguus;Morphine ;

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引用本文：

张敏, 聂琳, 刘磊, Wang Y T, Neuman R S, Bieger D. 兴奋性氨基酸介导的膜电流可被吗啡阻断[J]. 生理学报 1995; 47 (3): .

Zhang Min, Nie Lin, Liu Lei, Wang Y T, Neuman R S, Bieger D. Morphine blocked the exitatory amino acid mediated membrane current in ambigual motoneurons of the rat. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).