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大鼠中脑腹侧被盖区在睡眠-觉醒调节中的作用

张文慧, 钱朝霞, 汪海宏, 蔡葵, 林殷利

上海第二医科大学生理教研室. 上海 200025

摘要

31只清醒自由行动的雄性SD大鼠双侧中脑腹侧被盖区(VTA)微量注射3.3nmol溴隐亭后第2-3h觉醒时间显著增加(P<0.01); 6.6nmol溴隐亭有类似效果; 0.66后1.33nmol溴隐亭无明显 作用。同样方法VTA微量注射2nmol和4nmolSCH23390后第2-3h觉醒时间明显减少(分别为P<0.01和P<0.05), 但注射3.4nmol舒必利则无效。红藻氨酸(0.3#mu#g)双侧VTA注后一 wk, 大鼠白天觉醒减少, 夜间无明显变化。以上结果表明溴隐亭微量注射到VTA可能通过D_(1)受体使多巴胺神经元活动增加而产生致觉醒作用; 另外VTA中DA神经元对于维持日间 的觉醒可能有紧张性作用。图3表1参10

关键词： 中脑腹侧被盖区; 睡眠－觉醒周期; 微量注射; 多巴胺神经元; 溴隐亭; 红藻氨酸

Regulatory effect of ventral tegmental area on sleep-wakefulness in rats

Zhang Wenhui, Qian Zhaoxia, Wang Haihong, Cai Kui, Lin Yinli

Department of Physiology, Shanghai Second Medical University. Shanghai 200025

Abstract

Experiments were performed on 31 male SD rats. The results were as follows: (1)The time of waking was increased during the second and third hours after microinjection of 3.3 and 6.6 nmol bromocriptine into bilateral VTA (P<0.01), but the 1.33 nmol the second and third hours after microinjection of 2 and 4 nmol SCH23390 into VTA (P<0.01 and P<0.05 respectively), while 3,4 nmol sulpiride group was with--out effect. (3) One week after administration of kainic acid (0.3#mu#g), the rats presented less time of waking in the day (P<0.05), while sellp--wakefulness cycle at night showed no change. The resulsts suggest that bromocriptine could increase wakefulness by activating VTA DA neurons via D_(1) receptor and that the DA neurons might exert a tonic effect for maintaining the wakefulness at the daytime.

Key words: Ventral tegmental area;Sleep-wakefulness cycle;Microinjection;Dopamine neuron;Bromocriptine;Kainic acid

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引用本文：

张文慧, 钱朝霞, 汪海宏, 蔡葵, 林殷利. 大鼠中脑腹侧被盖区在睡眠-觉醒调节中的作用[J]. 生理学报 1995; 47 (2): .

Zhang Wenhui, Qian Zhaoxia, Wang Haihong, Cai Kui, Lin Yinli. Regulatory effect of ventral tegmental area on sleep-wakefulness in rats. Acta Physiol Sin 1995; 47 (2): (in Chinese with English abstract).