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甘蔗和烟草叶原生质体分离期间的膜损伤及有关酶活性变化

何若天, 覃伟, 李任强

广西农业大学实验中心. 广西,南宁(530005)

摘要

就甘蔗(Saccharum officinarum和烟草Nicotiana tabacum叶原生质体酶解分离期间 的O~(.)_(2)、膜脂过氧化、膜透性及某些能清除自由基的酶活性变化等对 膜受损原因作了探讨。(S5)图5表1参14

关键词： 原生质体; 超氧阴离子自由基[O_(2)~(-)]; 膜损伤; 甘蔗; 烟草

The membrane damage and changes of some related enzyme activities in protoplasts of sugarcane and tobacco during enzymatic isolation of leaf protoplsts

He Ruotian, Qin Wei, Li Renqiang

Research Centre, Guangxi Agricultural University. Nanning,Guangxi

Abstract

During the isolation of mesophyll protoplasts of sugarcane(saccharum officinarum)and tobacco(Nicotiana tabacum)by cell wall degrading enzyme mixture (1.5% cellulase, 0.5% pectinase and in 0.5mol/L of mannitol), the contents of O_(2) in protoplasts (containing some undigested cells) of both species increased gradually, and the level of malondialdehybe (MDA), as an index of peroxidation of membrane lipids, also increased in protoplasts of both species. The electrolyte leakage from freshly isolated protoplasts of both species were markedly higher than intact leaf tissue. The activities of superoxide dismutase (SOD) and peroxidase (POD) in protoplasts of both species increased gradually in varying degrees (Fig.3 and 4), and the activity of catalase (CAT) remained unchanged during enzymatic isolation of leaf protoplasts. In comparison with that in tobacco protoplasts, the content of O_(2) in sugarcane protoplasts was far higher but the acticities of SOD, POD and CAT were lower. The level of MDA in and the electrolytes leakafe from sugarcane protoplasts was markedly higher than tobacco proto-plasts. The above results showed that membrane damage of leaf protoplasts of sugarcane was...

Key words: Protoplast;Superoxide anion free radical[O_(2)~(-)];Membrane damage;Sugarcane;Tobacco

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引用本文：

何若天, 覃伟, 李任强. 甘蔗和烟草叶原生质体分离期间的膜损伤及有关酶活性变化[J]. 生理学报 1994; 46 (1): .

He Ruotian, Qin Wei, Li Renqiang. The membrane damage and changes of some related enzyme activities in protoplasts of sugarcane and tobacco during enzymatic isolation of leaf protoplsts. Acta Physiol Sin 1994; 46 (1): (in Chinese with English abstract).