过刊浏览

杜氏盐藻渗透调节过程中的甘油代谢途径

李红萍, 焦新之

中科院上海植物生理研究所. 上海(200032)

摘要

低渗处理提高杜氏盐藻细胞的呼吸速率60%以上; 高渗处理对呼吸无明显影响,但大大刺激光合放氧速率。呼吸链的细胞色素电子传递链抑制剂KCN和交替氧化酶抑制剂SHAM时杜氏藻渗 透调节过程中的呼吸、胞内甘油、ATP、淀粉含量的变化有不同的抑制效果。并探讨了低渗 与高渗情况下, 物质与能量代谢的变化及相互关系。图8表1参20

关键词： 杜氏藻; 渗透调节; 甘油; 氰化钾; 水杨基羟肟酸(SHAM)

Changes in intracellular glycerol content during osmoregulation in unicellular green alga Dunaliella salina

Li Hongping, Jiao Xinzhi

Shanghai Institute of Plant Physiology, Academia Sinica. Shanghai

Abstract

Intracellular glycerol content of D. salina increased after hyperosmotic shock and decreased after hypoosmotic shock. The respiration of algal cells was highly stimulated by hypoosmotic shock, oxygen uptake per cell increased by 60% or more. Hyperosmotic shock stimulated the respiration of algal cell much less than hypoosmotic shock. KCN 1 mmol/L and SHAM 2 mmol/L inhibited the change of respiration and the contents of intracellular glycerol, ATP and starch during osmoregulation caused by hypoosmotic shock, but they had more complicated effects on the metabolism during hyperosmotic regulation. It is concluded that during the adaptation to hypoosmotic shock the energy needed for glycerol degradation is supplied by both normal respiration pathway(KCN sensitive) and alternative pathway(SHAM sensitive); while that during the adaptation to hyperosmotic shock is supplied by normal pathway in darkness, and mainly by electron transport chain in light. During hypoosmotic regulation intracellular starch content increased and the glycerol content decreased, during hyperosmotic regulation starch sontent decreased while glycerol content increased.

Key words: Dunaliella salina;Osmoregulation;Glycerol;KCN;Salicylhydroxamic acid

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

李红萍, 焦新之. 杜氏盐藻渗透调节过程中的甘油代谢途径[J]. 生理学报 1994; 46 (1): .

Li Hongping, Jiao Xinzhi. Changes in intracellular glycerol content during osmoregulation in unicellular green alga Dunaliella salina. Acta Physiol Sin 1994; 46 (1): (in Chinese with English abstract).