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小麦叶绿体中细胞分裂素结合蛋白的功能

张华敏, 刘愚, 沈允钢

中科院上海植物生理研究所. 上海(200032)

摘要

小麦叶绿体经CTK结合蛋白的抗体处理后, 表现出: 希尔反应活力降低; MV(包括光系统I和 Ⅱ)和PMS(包括光系统Ⅰ)系统的毫秒延迟发光的慢相强度下降; 光合磷酸化活力受到抑制。说明小麦叶绿体中的CTK结合蛋白存在状态的改变会影响叶绿体的能量转换过程。图4表3参19

关键词： CTK结合蛋白; 抗体; 希尔反应; 毫秒延迟发光; 光合磷酸化

Study on the function of cytokinin-binding protein in wheat chloroplasts

Zhang Huamin, Liu Yu, Shen Yungang

Shanghai Institute of Plant Physiology. The Chinese Academy of Sciences. Shanghai

Abstract

Cytokinin-binding protein(CBP) was isolated from chloroplasts of wheat leaves and purified to a single protein by affinity chromatography on a BA-sepharose 6B column and then used as antigen. The rabbit antisera prepared contained antibodies against the CBP as judged by double-immuno-diffusion test. When chloroplasts were treated with the antiserum against CBP, Hill reaction activity was inhibited and the intensity of the slow phase of millisecond delayed light emission (msDLE) was reduced. The activity of photophosphorylation was also remarkably inhibited. It is suggested that CBP may affect the energy transduction process of chloroplasts.

Key words: CTK--binding protein;Antiserum;Hill reaction;msDLE;Photophosphorylation

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引用本文：

张华敏, 刘愚, 沈允钢. 小麦叶绿体中细胞分裂素结合蛋白的功能[J]. 生理学报 1994; 46 (4): .

Zhang Huamin, Liu Yu, Shen Yungang. Study on the function of cytokinin-binding protein in wheat chloroplasts. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).