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小球藻Rubis CO的纯化及其在异养向自养转变过程中的含量变化

张大兵, 李立人, 缪有刚, 吴庆金

南京大学生物系. 江苏, 南京；中科院上海植物生理研究所植物分子遗传国家重点实验室. 上海(200032)

摘要

报告了小球藻Rubis CO的分离纯化及其某些分子特性, Rubis CO在自养和异养小球藻细胞中的差异, 以及小球藻细胞由异养向自养转化过程中Rubis CO含量的变化, 在翻译水平上反映了Rubis CO基因表达受培养条件的调节和控制。图7表2参8

关键词： 小球藻; RubisCO; 纯化; 同源性; 自养; 异养

Purification of Rubis CO from Chlorella protothecoides and its content change during transition from hetertrophy to autotrophy

Zhang Dabin, Li Liren, Miao Yougang, Wu Qingyu

Department of Biology, Nanjing University. Nanjing, Jiangsu；China(200023)

Abstract

The RubisCO (E. C. 4. 1. 1. 39) from Chlorella protothecoides was purified by ammonium sulfate fractionation and column chromatography on Sephacryl S-300 and DEAE-cellulose. The recovery was 15%. The specific activity of the enzyme was 1.232 #mu#mol CO_(2)mg~(-1) protein min~(-1). Its molecular weight was 500 kD. The molecular weight, subunit constitution and immunoreactivity of the enzyme were similar to those from spinach leaves. The content of RubisCO in total soluble protein in autotrophic C. protothecoides cells was 24%. No RubisCO was found in heterotrophic C. protothecoides cells. RubisCO appeared in the C. protothecoides cells 24 h after transition from heterotrophy to autotrophy. Then it kept increasing until 41 h. Chlorophyll appeared 20 h after transition from heterotrophy to autotrophy.

Key words: Chlorella protothecoides;RubisCO;Purification;Homologous properties; Autotrophy;Heterotrophy

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引用本文：

张大兵, 李立人, 缪有刚, 吴庆金. 小球藻Rubis CO的纯化及其在异养向自养转变过程中的含量变化[J]. 生理学报 1994; 46 (4): .

Zhang Dabin, Li Liren, Miao Yougang, Wu Qingyu. Purification of Rubis CO from Chlorella protothecoides and its content change during transition from hetertrophy to autotrophy. Acta Physiol Sin 1994; 46 (4): (in Chinese with English abstract).