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低浓度氢清除法测定大鼠局部脑血流量

钟凯声, 吕敏, 顾正中

中科院上海生理研究所. 上海(200031)

摘要

采用低于爆炸范围下限的3%H_(2)浓度, 已可以与任何比例的氮氧混合气体安全混合, 且可避免吸纯氢期间的短暂缺氧, 更为严格地控制了实验条件, 增加了安全和准确性。对吸100%H_(2)和吸3%H_(2)以及吸3%H_(2)1—1.5min和吸3%H_(2) 5min测得脑血流量分别作了比较, 结果表明短期低浓度氢清除法能较好地反映动物的局部脑血流量。图2表2参10

Measurement of rCBF by hydrogen gas clearance with lower H_(2) concentration in rats

Zhong Kaisheng, Lu Min, Gu Zhengzhong

Shanghai Institute of Physiology, Chinese Academy of Sciences. Shanghai

Abstract

It was found that 3% H_(2) which is lower than the lower concentration limit guarded against occurrence of explosion could be mixed with any concentration ratio of N_(2)to O_(2) without causing transient hypoxia as ofthe found by inhaling 100% H_(2) and thus made clearance measurement more accurate and safe. Comparison studies between inhaling 100% H_(2) and 3% H_(2) as well as inhaling 3% H_(2) for 1-1.5 min and 3% H_(2) for 5min were undertaken. Repeated measurements of 3% H_(2) clearance for 1.5 min further showed that celarance test using low H_(2) concentration for short term periods reflected better the rCBF of experimental animals.

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引用本文：

钟凯声, 吕敏, 顾正中. 低浓度氢清除法测定大鼠局部脑血流量[J]. 生理学报 1994; 46 (3): .

Zhong Kaisheng, Lu Min, Gu Zhengzhong. Measurement of rCBF by hydrogen gas clearance with lower H_(2) concentration in rats. Acta Physiol Sin 1994; 46 (3): (in Chinese with English abstract).